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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Intrinsic tritium labeling of the capsular polysaccharide antigen of Haemophilus influenzae type B.

The capsular polysaccharide of Haemophilus influenzae type b was intrinsically labeled with tritium by a microculture technique with 6-3H-D-glucose and was isolated in radioantigenically pure form by a combination of selective precipitation and molecular sieve chromatography. Labeling with tritated sugar residues approached one-fourth maximum and produced a specific activity 10-fold that previously described for extrinsic labeling methods. In radioantigen-binding assays for antibody, sensitivity depended on the size of the antigen; preparations were readily made that could detect 0.01 microgram Ab/ml in serum samples of 25 microliter. Stability of the labeled antigen appears limited only by the primary radiodecomposition of tritium.[1]


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