Preparation and characterization of fibrinogen-coated, reversibly adhesive, lecithin/cholesterol vesicles.
We have developed a method for producing fibrinogen-coated, reversibly adhesive, lecithin/cholesterol vesicles. In this method, fibrinogen, which is acylated in the presence of preformed vesicles, spontaneously incorporates into vesicular membranes. The degree of incorporation is a function of the extent of acylation of the protein. Fibrinogen-coated vesicles aggregate in the presence of thrombin, a consequence of intervesicular fibrin formation. The rate and extent of thrombin-initiated aggregation depend on the fibrinogen surface concentration. Once aggregated, fibrin-coated vesicles can be dissociated by plasmin and by agents that disrupt intervesicular fibrin dimerization such as heparin and the tetrapeptide Gly-Pro-Arg-Pro. Fibrinogen-coated vesicles can be made to bind avidly to the surface of solution phase fibrin matrices and can be incorporated into solution phase fibrin clots. Fibrinogen-coated vesicles also bind to activated platelets. We propose that fibrinogen-coated vesicles will have practical applications as biomimetic hemostatic agents and as vehicles for the fibrin-specific targeting of drugs and other molecules.[1]References
- Preparation and characterization of fibrinogen-coated, reversibly adhesive, lecithin/cholesterol vesicles. DeAnglis, A.P., Retzinger, G.S. Journal of pharmaceutical sciences. (1995) [Pubmed]
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