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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Cloning and restriction mapping of the L-sorbose utilization genes from a clinical isolate of Escherichia coli (1).

About 30% of clinical isolates of Escherichia coli tested utilized L-sorbose as a carbon and energy source. Escherichia coli K-12 is naturally sorbose negative. The genes for L-sorbose utilization (sor+) is being used as a prototype for studying variable genes amongst bacterial pathogens. The sor+ genes from seven isolates were transferable to E. coli K-12. The (sor+) region was cloned into plasmid pBR322 to give pDOK1. Plasmid pDOK1 is approximately 20kb in size. A restriction endonuclease map of pDOK1 is presented.[1]


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