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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin.

Gender identification of forensic samples was determined by amplifying a segment of the X-Y homologous gene amelogenin. Using a single pair of primers spanning part of the first intron, 106 bp and 112 bp PCR products were generated from the X and Y homologues respectively, which were then resolved by agarose gel electrophoresis. This test enabled as little as 20 pg of DNA from severely degraded bones to be amplified and typed in a single tube reaction. Furthermore, using dye-labelled primers, it was possible to quantitate, by automated fluorescence detection, the relative yields of X and Y-specific PCR products generated from mixtures of male and female DNA. The versatility of this sex test was further demonstrated by co-amplifying with the HLA-DQA1 Amplitype kit in a combined gender/identity DNA test.[1]

References

  1. Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin. Mannucci, A., Sullivan, K.M., Ivanov, P.L., Gill, P. Int. J. Legal Med. (1994) [Pubmed]
 
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