Heavy chain-producing variants of a mouse myeloma cell line.
Eleven variants producing only heavy (H) chains have been isolated from the P3 cell line after mutagenesis with either ICR-191 or nitrosoguanidine. The H chains produced by these variants are similar both in molecular weight and by peptide maps with the parental H chain. H chains dimerize intreacellularly, are not secreted but persist as stable cytoplasmic proteins. No evidence for the continued synthesis of light (L) chains of L chain fragments has been found in these variants. These studies show that, in the P3 cell line, the synthesis of H chains can continue in the absence of detectable L chain synthesis.[1]References
- Heavy chain-producing variants of a mouse myeloma cell line. Morrison, S.L., Scharff, M.D. J. Immunol. (1975) [Pubmed]
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