Structure, genetic mapping, and expression of the mouse Hgf/scatter factor gene.
The cytokine termed hepatocyte growth factor or scatter factor ( HGF/ SF) has been implicated in embryonic development and liver regeneration. Mouse HGF/ SF cDNA clones were obtained by screening a mouse liver cDNA library with synthetic oligonucleotides, as well as by using the method of reverse transcription-polymerase chain reaction (RT-PCR) with mRNA isolated from adult mouse brain. Sequence analysis of mouse HGF/ SF cDNA clones spanning the entire coding region revealed an overall amino acid identity of 90% and 98% with human and rat HGF, respectively. Using mouse HGF antisense RNA as a probe, we observed the expression of HGF/ SF mRNA in the liver, kidney, whole brain and cerebellum of adult mice as well as in day 9 and day 10 mouse embryos. The mouse HGF/ SF cDNA was utilized for mapping Hgf to the centromeric region of mouse Chromosome 5 in apparent close proximity to the reeler mutation by the analysis of two multilocus crosses. The relationship of Hgf to the reeler mutation is discussed. The availability of mouse HGF/ SF cDNA clones should facilitate further analysis of HGF/ SF function during development.[1]References
- Structure, genetic mapping, and expression of the mouse Hgf/scatter factor gene. Lee, C.C., Kozak, C.A., Yamada, K.M. Cell Adhes. Commun. (1993) [Pubmed]
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