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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A technique for preparing human dermal and scar specimens for scanning electron microscopy.

The aims in a current study of dermis and scar tissue using the scanning electron microscope are: (1) to maintain the orientation of the specimen, (2) to preserve the in vivo architecture, and (3) to expose a satisfactory surface. A technique which best fulfils these requirements involves glutaraldehyde fixation of a splinted specimen, freeze-fracturing using a modified pair of pliers, freeze-drying again after washing. Consistently satisfactory specimens have been obtained, which provide information about fibre architecture and cell distribution.[1]

References

  1. A technique for preparing human dermal and scar specimens for scanning electron microscopy. Sommerlad, B.C., Creasey, J.M. Journal of microscopy. (1975) [Pubmed]
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