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MeSH Review

Freeze Drying

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Disease relevance of Freeze Drying


High impact information on Freeze Drying

  • The peptide is inactivated by heating above 60 degrees C, but recovers full catalytic activity upon cooling and lyophilization from acetic acid [6].
  • Autoradiography of the stomach tissue was carried out by freeze-drying, embedding in Epon, wet-sectioning with ethylene glycol, and dry-mounting the emulsion film by the wire-loop method to prevent loss of the labeled substance [7].
  • Second, the technique of freeze-drying molecules on mica (Heuser, J. E., 1983, J. Mol. Biol., 169:155-195) was modified to overcome the low affinity of mica in 2 mM MES, by pretreating the mica with polylysine [8].
  • The general appearance and dimensions of heavy metal-free molecules were similar to those of shadowed myosin, either after freeze-drying without or air-drying with glycerol [9].
  • After freeze-drying at -80 degrees C (a method that is superior to air-drying), shadowing with tantalum/tungsten, and image processing, structural details on both surfaces are portrayed in the range of 2 nm [10].

Chemical compound and disease context of Freeze Drying


Biological context of Freeze Drying


Anatomical context of Freeze Drying


Associations of Freeze Drying with chemical compounds


Gene context of Freeze Drying

  • A few experiments examining lyophilization effects on steroid receptors from human breast biopsies did note some diminution of the 8S form of both ER and PgR following lyophilization, although this effect was variable in extent [31].
  • By lyophilization from 40% acetic acid solutions, bovine ribonuclease A forms several types of three-dimensional domain-swapped oligomers: dimers, trimers, tetramers, and higher order multimers [32].
  • Extensive oligomerization of the decorin protein core was observed only after dialysis against water and freeze-drying [33].
  • Dry, excipient-free recombinant human tissue-type plasminogen activator (tPA) powder was prepared by lyophilization from ammonium bicarbonate solution [34].
  • RESULTS: Mass ratios of sucrose to raffinose did not affect the recovery of G6PDH activity after freeze-drying, but significantly affected the stability of freeze-dried G6PDH during storage [35].

Analytical, diagnostic and therapeutic context of Freeze Drying


  1. Slowed enzymatic turnover allows characterization of intermediates by solid-state NMR. Studelska, D.R., McDowell, L.M., Espe, M.P., Klug, C.A., Schaefer, J. Biochemistry (1997) [Pubmed]
  2. Attenuated, streptomycin-dependent Salmonella typhi oral vaccine: potential deleterious effects of lyophilization. Levine, M.M., DuPont, H.L., Hornick, R.B., Snyder, M.J., Woodward, W., Gilman, R.H., Libonati, J.P. J. Infect. Dis. (1976) [Pubmed]
  3. Virulence and viability of Yersinia pestis 25 years after lyophilization. Heckly, R.J., Blank, H. Appl. Environ. Microbiol. (1980) [Pubmed]
  4. Involvement of cyclopropane fatty acids in the response of Pseudomonas putida KT2440 to freeze-drying. Muñoz-Rojas, J., Bernal, P., Duque, E., Godoy, P., Segura, A., Ramos, J.L. Appl. Environ. Microbiol. (2006) [Pubmed]
  5. Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying. Leslie, S.B., Israeli, E., Lighthart, B., Crowe, J.H., Crowe, L.M. Appl. Environ. Microbiol. (1995) [Pubmed]
  6. Design and synthesis of a peptide having chymotrypsin-like esterase activity. Hahn, K.W., Klis, W.A., Stewart, J.M. Science (1990) [Pubmed]
  7. Autoradiographic demonstration of gastrin-releasing peptide-binding sites in the rat gastric mucosa. Nakamura, M., Oda, M., Kaneko, K., Akaiwa, Y., Tsukada, N., Komatsu, H., Tsuchiya, M. Gastroenterology (1988) [Pubmed]
  8. Deep-etch visualization of 27S clathrin: a tetrahedral tetramer. Heuser, J.E., Keen, J.H., Amende, L.M., Lippoldt, R.E., Prasad, K. J. Cell Biol. (1987) [Pubmed]
  9. Unshadowed myosin molecules: STEM mass-maps of myosin heads. Walzthöny, D., Bähler, M., Eppenberger, H.M., Wallimann, T., Engel, A. EMBO J. (1984) [Pubmed]
  10. Single bacteriorhodopsin molecules revealed on both surfaces of freeze-dried and heavy metal-decorated purple membranes. Studer, D., Moor, H., Gross, H. J. Cell Biol. (1981) [Pubmed]
  11. A new method for the purification of 30S ribosomal proteins from Escherichia coli using nondenaturing conditions. Littlechild, J.A., Malcolm, A.L. Biochemistry (1978) [Pubmed]
  12. Removal/neutralization of hepatitis A virus during manufacture of high purity, solvent/detergent factor VIII concentrate. Lemon, S.M., Murphy, P.C., Smith, A., Zou, J., Hammon, J., Robinson, S., Horowitz, B. J. Med. Virol. (1994) [Pubmed]
  13. Immunoelectron microscopic localization of sarcoplasmic reticulum proteins in cryofixed, freeze-dried, and low temperature-embedded tissue. Jorgensen, A.O., McGuffee, L.J. J. Histochem. Cytochem. (1987) [Pubmed]
  14. Solid-state characteristics of amorphous sodium indomethacin relative to its free acid. Tong, P., Zografi, G. Pharm. Res. (1999) [Pubmed]
  15. Mutagenicity induced by lyophilization or storage of urine from isoniazid-treated rats. Miller, C.T., Stoltz, D.R. Mutat. Res. (1978) [Pubmed]
  16. Preparation of allosteric ribonuclease. Walker, E.J., Ralston, G.B., Darvey, I.G. Biochem. J. (1978) [Pubmed]
  17. The structure of partly decondensed metaphase chromosomes. Nasedkina, T.V., Slesinger, S.I. Chromosoma (1982) [Pubmed]
  18. Second derivative-high-performance liquid chromatographic-fluorometric detection of porphyrins in chick embryo liver cell culture medium. Zelt, D.T., Owen, J.A., Marks, G.S. J. Chromatogr. (1980) [Pubmed]
  19. X-ray absorption spectroscopy of soybean lipoxygenase-1. Influence of lipid hydroperoxide activation and lyophilization on the structure of the non-heme iron active site. Van der Heijdt, L.M., Feiters, M.C., Navaratnam, S., Nolting, H.F., Hermes, C., Veldink, G.A., Vliegenthart, J.F. Eur. J. Biochem. (1992) [Pubmed]
  20. Novel chitosan/collagen scaffold containing transforming growth factor-beta1 DNA for periodontal tissue engineering. Zhang, Y., Cheng, X., Wang, J., Wang, Y., Shi, B., Huang, C., Yang, X., Liu, T. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
  21. Avian tibial dyschondroplasia. III. Electron probe analysis. Hargest, T.E., Gay, C.V., Leach, R.M. Am. J. Pathol. (1985) [Pubmed]
  22. Tolerance of the mouse sperm nuclei to freeze-drying depends on their disulfide status. Kaneko, T., Whittingham, D.G., Overstreet, J.W., Yanagimachi, R. Biol. Reprod. (2003) [Pubmed]
  23. Localization of a mosquito-larval toxin of Bacillus sphaericus 1593. Myers, P.S., Yousten, A.A. Appl. Environ. Microbiol. (1980) [Pubmed]
  24. The effects of freeze-drying and ethylene oxide sterilization on the mechanical properties of human patellar tendon. Bechtold, J.E., Eastlund, D.T., Butts, M.K., Lagerborg, D.F., Kyle, R.F. The American journal of sports medicine. (1994) [Pubmed]
  25. Porous chitosan scaffold containing microspheres loaded with transforming growth factor-beta1: implications for cartilage tissue engineering. Kim, S.E., Park, J.H., Cho, Y.W., Chung, H., Jeong, S.Y., Lee, E.B., Kwon, I.C. Journal of controlled release : official journal of the Controlled Release Society. (2003) [Pubmed]
  26. alpha-lytic protease can exist in two separately stable conformations with different His57 mobilities and catalytic activities. Haddad, K.C., Sudmeier, J.L., Bachovchin, D.A., Bachovchin, W.W. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  27. Steroidogenic potential of lyophilized mitochondria from bovine adrenocortical tissue. Prasad, V.V., Mathur, C., Welch, M., Lieberman, S. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  28. Preservation of metabolic activity in lyophilized human erythrocytes. Goodrich, R.P., Sowemimo-Coker, S.O., Zerez, C.R., Tanaka, K.R. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  29. Calcification of isolated matrix vesicles and reconstituted vesicles from fetal bovine cartilage. Hsu, H.H., Anderson, H.C. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  30. Low-density lipoprotein as a carrier of antitumoral drugs: in vivo fate of drug-human low-density lipoprotein complexes in mice. Masquelier, M., Vitols, S., Peterson, C. Cancer Res. (1986) [Pubmed]
  31. The effect of lyophilization on steroid receptor behavior, as defined by sucrose density gradient analysis. Janes, G.R., Koenders, A.J., Benraad, T.J. J. Steroid Biochem. (1982) [Pubmed]
  32. Oligomerization of ribonuclease A: two novel three-dimensional domain-swapped tetramers. Gotte, G., Libonati, M. J. Biol. Chem. (2004) [Pubmed]
  33. Biologically active decorin is a monomer in solution. Goldoni, S., Owens, R.T., McQuillan, D.J., Shriver, Z., Sasisekharan, R., Birk, D.E., Campbell, S., Iozzo, R.V. J. Biol. Chem. (2004) [Pubmed]
  34. Preparation of excipient-free recombinant human tissue-type plasminogen activator by lyophilization from ammonium bicarbonate solution: an investigation of the two-stage sublimation phenomenon. Overcashier, D.E., Brooks, D.A., Costantino, H.R., Hsu, C.C. Journal of pharmaceutical sciences. (1997) [Pubmed]
  35. Effect of sucrose/raffinose mass ratios on the stability of co-lyophilized protein during storage above the Tg. Davidson, P., Sun, W.Q. Pharm. Res. (2001) [Pubmed]
  36. Solid-phase methods for sequencing of nucleic acids I. Simultaneous sequencing of different oligodeoxyribonucleotides using a new, mechanically stable anion-exchange paper. Rosenthal, A., Schwertner, S., Hahn, V., Hunger, H.D. Nucleic Acids Res. (1985) [Pubmed]
  37. Expression of human chorionic gonadotropin uniformly labeled with NMR isotopes in Chinese hamster ovary cells: an advance toward rapid determination of glycoprotein structures. Lustbader, J.W., Birken, S., Pollak, S., Pound, A., Chait, B.T., Mirza, U.A., Ramnarain, S., Canfield, R.E., Brown, J.M. J. Biomol. NMR (1996) [Pubmed]
  38. Cytosine and orotic acid in urine of immunodeficient children. Mills, G.C., Schmalstieg, F.C., Newkirk, K.E., Goldblum, R.M. Clin. Chem. (1979) [Pubmed]
  39. 31P NMR of tissue phospholipids: a comparison of three tissue pre-treatment procedures. Meneses, P., Para, P.F., Glonek, T. J. Lipid Res. (1989) [Pubmed]
  40. Expression of hormone-sensitive lipase and its regulation by adrenaline in skeletal muscle. Langfort, J., Ploug, T., Ihlemann, J., Saldo, M., Holm, C., Galbo, H. Biochem. J. (1999) [Pubmed]
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