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Li, X.Y. et al.

An improved method for the measurement of malondialdehyde in biological samples.

An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50 degrees C for 20 min, and then with 0.4% TBA at 60 degrees C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high-performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.[1]

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An improved method for the measurement of malondialdehyde in biological samples. Li, X.Y., Chow, C.K. Lipids (1994) [Pubmed]

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