Immunoassay of podophyllotoxin.
The purpose of our research was to design and develop a sensitive immunoassay for the natural Podophyllum lignan, podophyllotoxin [1], which is the precursor of the anti-cancer drugs etoposide [2] and teniposide [3]. In order to obtain a specific antiserum, bovine serum albumin and keyhole limpet hemocyanin conjugates of succinyl-podophyllotoxin [5] were prepared as antigenic hapten carrier proteins. These antigens were used to develop polyclonal antibodies to 1 in rabbits. The antisera were used for the development of an immunoassay for the detection of 1 and its 4'-demethyl derivative 4. As little as 0.16 nmol of 1 is detectable with this ELISA. The assay was applied to the direct analysis of 1 in plants for the screening of potential drug plants which allowed quantitation in less than 0.2 mg of plant tissue.[1]References
- Immunoassay of podophyllotoxin. Yoo, K., Porter, J.R. J. Nat. Prod. (1993) [Pubmed]
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