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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Sequence of extracellular mouse protein BM-90/ fibulin and its calcium-dependent binding to other basement-membrane ligands.

Partial sequence comparisons have recently indicated that two extracellular components, fibulin from human placenta and BM-90 from a basement-membrane-producing mouse tumor, are either identical or closely related proteins. In this study, a complete sequence analysis of mouse BM-90 cDNA showed a 539-residue N-terminal core structure (domains I and II), which was 85% identical with the same core structure of human fibulin. A 137-residue C-terminal sequence (domain III) was unique for BM-90 and could also be identified by Edman degradation. This suggested a novel splice product, variant D, which is characteristic for the mouse tumor. A second 117-residue C-terminal sequence (domain III) was identified in additional mouse cDNA clones and showed 91% identity with the region specific for variant C of fibulin. Northern blots using mouse cells demonstrated two mRNA species, 2.7 kb and 2.3 kb, which encoded the variants D and C, respectively. The sequence of BM-90/ fibulin indicates the presence of nine epidermal-growth-factor-like repeats in the core domain-II structure, eight of which contain consensus motifs for calcium binding. This binding is apparently important for the interaction of BM-90 with laminin and nidogen and for some weaker interactions with collagen IV. Further binding of BM-90 was demonstrated to fibronectin and BM-90 itself, but did not depend on calcium. Major binding sites for BM-90 were identified at a C-terminal segment of laminin A chain and at the N-terminus of nidogen. The broad interaction repertoire of BM-90 is comparable to that of nidogen and both proteins may have similar roles as connecting elements in the extracellular matrix.[1]


  1. Sequence of extracellular mouse protein BM-90/fibulin and its calcium-dependent binding to other basement-membrane ligands. Pan, T.C., Kluge, M., Zhang, R.Z., Mayer, U., Timpl, R., Chu, M.L. Eur. J. Biochem. (1993) [Pubmed]
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