Disease relevance of Lama1

• Modulation of murine B16F10 melanoma plasminogen activator production by a synthetic peptide derived from the laminin A chain [1].
• Messenger RNA levels for ECM genes except for laminin A chain increased significantly with the progression of nephritis in NZB/W F1 mice [2].
• Compared with PYS-2 cells, a mouse teratocarcinoma cell line which constitutively expresses a 10-kb mRNA transcript for 'classical' laminin A-chain, cultured vascular smooth muscle cells (VSMC) did not express a corresponding mRNA [3].
• Identification of an amino acid sequence from the laminin A chain that stimulates metastasis and collagenase IV production [4].
• Rat monoclonal antibodies were raised against fragment E3 of the mouse Engelbreth-Holm-Swarm (EHS) tumor laminin and selected according to their exclusive reaction with laminin A chain by immunoblotting and staining pattern in embryonic kidneys by immunofluorescence [5].

High impact information on Lama1

• Transient and locally restricted expression of laminin A chain mRNA by developing epithelial cells during kidney organogenesis [6].
• Immunofluorescence staining of embryonic kidneys and of organ cultures of metanephric mesenchyme demonstrated coappearance of the integrin alpha 6 subunit and the laminin A-chain in regions where nonpolarized mesenchymal cells convert into polarized epithelial cells [7].
• In some other cell types (endothelium, some ureter cells) an exclusive expression of alpha 6 with no apparent colocalization of laminin A-chain in the corresponding basement membrane was seen [7].
• Mutagenic insertions in both Lama1 and Lamb1 were obtained in a secretory gene trap screen [8].
• Overexpression of laminin alpha5 from a transgene improved the phenotype of Lama1(-/-) embryos to the point that they initiated gastrulation, but this overexpression did not rescue Reichert's membrane, and trophoblast cells did not form blood sinuses [8].

Biological context of Lama1

• In the present study, we further showed that SOX7 and SOX17 are involved in the regulation of parietal endoderm-specific enhancer activity of the mouse Lama1 gene [9].
• To understand how laminin alpha1 chain expression is regulated, we cloned and characterized a 2-kb promoter region of the Lama1 mouse gene [10].
• Major binding sites for BM-90 were identified at a C-terminal segment of laminin A chain and at the N-terminus of nidogen [11].
• We have determined that the mouse laminin B2-chain gene is located on chromosome 1 (confirming this assignment) and the laminin A-chain gene is located on chromosome 17 [12].
• Laminin A chain polypeptides showed a much more restricted tissue distribution than the B chains [13].

Anatomical context of Lama1

• 5. However, in Lama1(-/-) embryos, the embryonic basement membrane forms, the embryonic ectoderm cavitates and the parietal endoderm differentiates, apparently because laminin 10 (alpha5beta1gamma1) partially compensates for the absent laminin 1 [8].
• Using an 850-base cDNA probe for the mouse laminin A-chain and a 1000-base cDNA probe for the mouse laminin B2 chain, we have screened mouse-Chinese hamster somatic cell hybrids in order to assign the genes for each of these polypeptides to their respective mouse chromosome [12].
• The RGD containing site of the mouse laminin A chain is active for cell attachment, spreading, migration and neurite outgrowth [14].
• The na-EAT cells (but not the a-EAT cells) also secrete laminin A chain into the culture medium; however, no B chain was detected in the culture medium of either cell line [15].
• Angiotensin-II-induced expression of laminin complex and laminin A-chain-related transcripts in vascular smooth muscle cells [3].

Associations of Lama1 with chemical compounds

• An overall similarity of location between alpha 6 integrin subunit and laminin A chain at the epithelial/stromal interface was noted [16].
• In this report, we demonstrate that the murine mast cell line PT18 and primary mouse bone marrow-derived mast cells chemotax to the basement membrane glycoprotein laminin, and that the synthetic laminin A chain-derived peptide, PA22-2, represents a region of laminin that contains a major chemoattractant site [17].
• The discrete elevation of laminin A chain mRNA level was also observed after estrogen stimulation in the delayed implantation model [18].
• Estrogenic stimulation to ovariectomized, progesterone-treated pregnant mice resulted in about a three-fold increase of laminin A chain mRNA levels [18].

Other interactions of Lama1

• Northern blot analysis revealed that the steady-state levels of mouse Sox7 and Sox17 mRNAs increased in parallel with that of Lama1 mRNA during the differentiation of F9 cells [9].
• Both SOX7 and SOX17 markedly trans-activated the transcription of the Lama1 enhancer-reporter construct in undifferentiated F9 cells in a manner dependent on high mobility group box-mediated DNA binding [9].
• Messenger RNA levels of beta-actin remained constant, and those of laminin A chain could not be detected in either control or diseased kidneys [2].
• Domain V contains three repeats with similarity to the laminin A chain G domain that are separated by epidermal growth factor-like regions not found in the laminin A chain [19].

Analytical, diagnostic and therapeutic context of Lama1

• Electrophoretic mobility shift assays and mutational analyses revealed that SOX7 and SOX17 bound specifically to two SOX-binding sites within the Lama1 enhancer, and that these SOX-binding sites functioned synergistically to confer the trans-activation by SOX7 and SOX17 [9].
• Lama is a PSP minigene and allows analysis of the PSP gene 5' regulatory region by transgenesis [20].
• Immune response of the llama (Lama glama) to tetanus toxoid vaccination [21].
• Lama glama (the South American camelid, llama): a unique model for evaluation of xenogenic islet transplants in a cerebral spinal fluid driven artificial organ [22].

References