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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA.

Seven hundred fifty-eight processed sputum sediments received for the diagnosis of tuberculosis or other mycobacterial infections were tested by utilizing a rRNA target amplification assay and traditional culture techniques. The results from the rRNA target amplification assay (Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test), available in 5 h, were compared with the results from standard culture techniques held for 6 weeks. A total of 119 specimens (16%) were culture positive for Mycobacterium tuberculosis. Overall sensitivity, specificity, positive predictive value, and negative predictive value were 82, 99, 97, and 96%, respectively, for the Gen-Probe assay; 88, 100, 100, and 97%, respectively, for culture; and 53, 99.8, 99.6, and 91%, respectively, for fluorochrome stain. The Gen-Probe assay employs the isothermal enzymatic amplification of M. tuberculosis complex rRNA followed by detection of the amplicon with an acridinium ester-labeled DNA probe. This assay has the potential of reducing the time for diagnosis of tuberculosis to 1 day.[1]

References

  1. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA. Jonas, V., Alden, M.J., Curry, J.I., Kamisango, K., Knott, C.A., Lankford, R., Wolfe, J.M., Moore, D.F. J. Clin. Microbiol. (1993) [Pubmed]
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