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MeSH Review

Culture Techniques

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Disease relevance of Culture Techniques


High impact information on Culture Techniques


Chemical compound and disease context of Culture Techniques


Biological context of Culture Techniques


Anatomical context of Culture Techniques


Associations of Culture Techniques with chemical compounds


Gene context of Culture Techniques

  • These results indicate that the panhematopoietic properties of TPO identified by in vitro culture techniques correlate well with its effects in animals [31].
  • Positivity was only restored in CD34-positive leukaemic cells using a modified culture technique in which cells were cultured as pre-formed small aggregates [32].
  • In contrast, cytokine secretion responses to IL-4 and IL-13 were highly dependent on the culture technique; cells cultured on plastic exhibited significant concentration-dependent increases in GM-CSF and TGF-beta(2) secretion, whereas cells grown at ALI showed no statistically significant response [33].
  • A duodenal biopsy culture technique was used to investigate the effect of substance P on lymphokine secretion by the human gut associated lymphoid tissue [34].
  • The in vitro culture technique will allow for further detailed studies of the interactions between the various cell populations and the Ts1 cell-derived soluble factor during the induction of Ts2 cells [35].

Analytical, diagnostic and therapeutic context of Culture Techniques


  1. Cytokines in chronic inflammatory arthritis. II. Granulocyte-macrophage colony-stimulating factor in rheumatoid synovial effusions. Xu, W.D., Firestein, G.S., Taetle, R., Kaushansky, K., Zvaifler, N.J. J. Clin. Invest. (1989) [Pubmed]
  2. Divergent patterns of marrow cell suspension culture growth in the myeloid leukemias: correlation of in vitro findings with clinical features. Elias, L., Greenberg, P. Blood (1977) [Pubmed]
  3. Hematopoietic toxicity by cimetidine. Reexamination using the antimetabolite azathioprine. Gamelli, R.L., Foster, R.S., Whisnant, J.K. Transplantation (1983) [Pubmed]
  4. The epidemiology of gentamicin-resistant Pseudomonas aeruginosa on an intermediate care unit. MacArthur, R.D., Lehman, M.H., Currie-McCumber, C.A., Shlaes, D.M. Am. J. Epidemiol. (1988) [Pubmed]
  5. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA. Jonas, V., Alden, M.J., Curry, J.I., Kamisango, K., Knott, C.A., Lankford, R., Wolfe, J.M., Moore, D.F. J. Clin. Microbiol. (1993) [Pubmed]
  6. Glucose induces intracisternal type A retroviral gene transcription and translation in pancreatic beta cells. Leiter, E.H., Fewell, J.W., Kuff, E.L. J. Exp. Med. (1986) [Pubmed]
  7. Induction of recombination activating gene expression in a human lymphoid progenitor cell line: requirement of two separate signals from stromal cells and cytokines. Tagoh, H., Kishi, H., Okumura, A., Kitagawa, T., Nagata, T., Mori, K., Muraguchi, A. Blood (1996) [Pubmed]
  8. Use of cell separation and short-term culture techniques to study erythroid cell development. Glass, J., Lavidor, L.M., Robinson, S.H. Blood (1975) [Pubmed]
  9. Antisense oligonucleotides against cytochrome P450 2C8 attenuate EDHF-mediated Ca(2+) changes and dilation in isolated resistance arteries. Bolz, S.S., Fisslthaler, B., Pieperhoff, S., De Wit, C., Fleming, I., Busse, R., Pohl, U. FASEB J. (2000) [Pubmed]
  10. Tissue culture of human fetal pancreas. Effects of nicotinamide on insulin production and formation of isletlike cell clusters. Sandler, S., Andersson, A., Korsgren, O., Tollemar, J., Petersson, B., Groth, C.G., Hellerström, C. Diabetes (1989) [Pubmed]
  11. Is penicillin G an adequate initial treatment for aspiration pneumonia? A prospective evaluation using a protected specimen brush and quantitative cultures. Mier, L., Dreyfuss, D., Darchy, B., Lanore, J.J., Djedaïni, K., Weber, P., Brun, P., Coste, F. Intensive care medicine. (1993) [Pubmed]
  12. HIV-1 sensitivity to zidovudine: a consensus culture technique validated by genotypic analysis of the reverse transcriptase. Brun-Vézinet, F., Ingrand, D., Deforges, L., Gochi, K., Ferchal, F., Schmitt, M.P., Jung, M., Masquelier, B., Aubert, J., Buffet-Janvresse, C. J. Virol. Methods (1992) [Pubmed]
  13. Dialysis culture for determination of MIC and MBC of benzylpenicillin against Borrelia burgdorferi. Stiernstedt, S.H., Tadesse, T., Wretlind, B. APMIS (1999) [Pubmed]
  14. Degradation and metabolism of hexazinone by two isolated bacterial strains from soil. Wang, X., Wang, H., Tan, C. Chemosphere (2005) [Pubmed]
  15. Metabolic diversity of aromatic hydrocarbon-degrading bacteria from a petroleum-contaminated aquifer. Mikesell, M.D., Kukor, J.J., Olsen, R.H. Biodegradation (1993) [Pubmed]
  16. Ecdysteroids accelerate mitoses in accessory glands of beetle pupae. Szopa, T.M., Rousseaux, J.J., Yuncker, C., Happ, G.M. Dev. Biol. (1985) [Pubmed]
  17. The role of chiropractic adjusting tables as reservoirs for microbial diseases. Bifero, A.E., Prakash, J., Bergin, J. American journal of infection control. (2006) [Pubmed]
  18. Evidence against a role for polymorphisms at tumor necrosis factor, interleukin-1 and interleukin-1 receptor antagonist gene loci in the regulation of disease severity in acute pancreatitis. Powell, J.J., Fearon, K.C., Siriwardena, A.K., Ross, J.A. Surgery (2001) [Pubmed]
  19. Organotypic rat cerebellar slice culture as a model to analyze the molecular pharmacology of GABAA receptors. Davids, E., Hevers, W., Dämgen, K., Zhang, K., Tarazi, F.I., Lüddens, H. European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology. (2002) [Pubmed]
  20. Evaluation of techniques for increasing mitotic yield and chromosome length in leukemic cells. Rapoza, P.A., Testa, J.R., Egues, M.C., Rowley, J.D. Acta Cytol. (1984) [Pubmed]
  21. Generation of T helper cells in vitro. IV. F1 T helper cells primed with antigen-pulsed parental macrophages are genetically restricted in their antigen-specific helper activity. McDougal, J.S., Cort, S.P. J. Immunol. (1978) [Pubmed]
  22. An improved lymphocyte culture technique: deoxycytidine release of a thymidine block and use of a constant humidity chamber for slide making. Wheater, R.F., Roberts, S.H. J. Med. Genet. (1987) [Pubmed]
  23. Androgenic stimulation of progesterone production by granulosa cells from preantral ovarian follicles: further in vitro studies using replicate cell cultures. Hillier, S.G., Knazek, R.A., Ross, G.T. Endocrinology (1977) [Pubmed]
  24. The effect of prolactin on human BPH epithelial cell proliferation. Syms, A.J., Harper, M.E., Griffiths, K. Prostate (1985) [Pubmed]
  25. Expression of p53 in urothelial cell cultures from tumour-bearing and tumour-free patients. Harney, J., Murphy, D.M., Jones, M., Mothersill, C. Br. J. Cancer (1995) [Pubmed]
  26. IL-5 secretion by allergen-stimulated CD4+ T cells in primary culture: relationship to expression of allergic disease. Till, S., Dickason, R., Huston, D., Humbert, M., Robinson, D., Larché, M., Durham, S., Kay, A.B., Corrigan, C. J. Allergy Clin. Immunol. (1997) [Pubmed]
  27. Clinical evaluation of a new recombinant antigen-based cytomegalovirus immunoglobulin M immunoassay in liver transplant recipients. Halling, V.W., Maine, G.T., Groettum, C.M., Wilson, J.A., Spesard, J., Brojanac, S., Combs, B., Wachta, D., Holas, C., Wilson, T., Wang, D., Tomazic-Allen, S., Lazzarotto, T., Landini, M.P., Jevremovic-Simovic, S., Wiesner, R.H., Paya, C.V., Smith, T.F. Transplantation (2001) [Pubmed]
  28. In vitro culture system to determine MICs and MBCs of antimicrobial agents against Treponema pallidum subsp. pallidum (Nichols strain). Norris, S.J., Edmondson, D.G. Antimicrob. Agents Chemother. (1988) [Pubmed]
  29. Analysis of the breakpoints in translocation (15;17) observed in 4 patients with acute promyelocytic leukemia. Hagemeijer, A., Löwenberg, B., Abels, J. Hum. Genet. (1982) [Pubmed]
  30. Microorganisms capable of metabolizing the herbicide metolachlor. Saxena, A., Zhang, R.W., Bollag, J.M. Appl. Environ. Microbiol. (1987) [Pubmed]
  31. Do the preclinical effects of thrombopoietin correlate with its in vitro properties? Kaushansky, K., Broudy, V.C., Sitnicka, E., Lofton-Day, C., Grossmann, A., Sprugel, K. Stem Cells (1996) [Pubmed]
  32. Immunostaining of whole agar cultures by APAAP. Burgess, R., Hyde, K., Lenehan, H., Brereton, M.L., Geary, C.G., Yin, J.A. Clinical and laboratory haematology. (1995) [Pubmed]
  33. Differentiation-dependent responsiveness of bronchial epithelial cells to IL-4/13 stimulation. Kikuchi, T., Shively, J.D., Foley, J.S., Drazen, J.M., Tschumperlin, D.J. Am. J. Physiol. Lung Cell Mol. Physiol. (2004) [Pubmed]
  34. Substance P modulates lymphokine activities in supernatants of cultured human duodenal biopsies. Hart, R., Dancygier, H., Wagner, F., Niedermeyer, H., Classen, M. Immunol. Lett. (1988) [Pubmed]
  35. Characterization of an in vitro-stimulated, Cryptococcus neoformans-specific second-order suppressor T cell and its precursor. Fidel, P.L., Murphy, J.W. Infect. Immun. (1988) [Pubmed]
  36. Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. Huijsdens, X.W., Linskens, R.K., Mak, M., Meuwissen, S.G., Vandenbroucke-Grauls, C.M., Savelkoul, P.H. J. Clin. Microbiol. (2002) [Pubmed]
  37. Application of flow cytometry and fluorescent in situ hybridization for assessment of exposures to airborne bacteria. Lange, J.L., Thorne, P.S., Lynch, N. Appl. Environ. Microbiol. (1997) [Pubmed]
  38. Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. Sherwood, E.R., Berg, L.A., Mitchell, N.J., McNeal, J.E., Kozlowski, J.M., Lee, C. J. Urol. (1990) [Pubmed]
  39. Mycobacteria and glutaraldehyde: is high-level disinfection of endoscopes possible? Urayama, S., Kozarek, R.A., Sumida, S., Raltz, S., Merriam, L., Pethigal, P. Gastrointest. Endosc. (1996) [Pubmed]
  40. Natural cycles for in-vitro fertilization: cost-effectiveness analysis and factors influencing outcome. Daya, S., Gunby, J., Hughes, E.G., Collins, J.A., Sagle, M.A., YoungLai, E.V. Hum. Reprod. (1995) [Pubmed]
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