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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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The NV genes of fish rhabdoviruses: development of RNase protection assays for rapid assessment of genetic variation.

The genomic RNA of the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV) contains a small NV gene which is not present in any other rhabdoviruses characterized to date. We have constructed a plasmid which carries a full length cDNA copy of the IHNV NV gene between 2 RNA polymerase promoters such that plus-sense and minus-sense RNA transcripts of the IHNV NV gene can be synthesized. These were used to develop an RNase protection assay which was capable of detecting as little as 1-5 ng of NV mRNA or genomic RNA. RNA from tissue culture cells infected with 3 different strains of IHNV showed distinct, diagnostic cleavage patterns reflecting sequence variation in their NV genes.[1]

References

  1. The NV genes of fish rhabdoviruses: development of RNase protection assays for rapid assessment of genetic variation. Kurath, G., Higman, K.H., Björklund, H.V. Vet. Res. (1995) [Pubmed]
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