Involvement of the Saccharomyces cerevisiae HDF1 gene in DNA double-strand break repair and recombination.
The HDF1 protein of Saccharomyces cerevisiae shares biochemical properties and structural homology with the 70-kDa subunit of the human autoantigen Ku. The Ku protein, a heterodimer composed of a 70-kDa subunit and an 80-kDa subunit, has been identified as the regulatory subunit of the DNA-dependent protein kinase. This enzyme has recently been shown to be involved in DNA repair and recombination processes in mammalian cells. Here we show that hdf1-disrupted S. cerevisiae strains are strongly sensitive toward the radiomimetic antibiotic bleomycin. In addition, mating-type switching and rates of spontaneous mitotic recombination are strongly reduced. This phenotype is similar to that of mammalian cells lacking components of the DNA-dependent protein kinase holoenzyme, suggesting that HDF1 participates in and exerts equivalent functions in S. cerevisiae.[1]References
- Involvement of the Saccharomyces cerevisiae HDF1 gene in DNA double-strand break repair and recombination. Mages, G.J., Feldmann, H.M., Winnacker, E.L. J. Biol. Chem. (1996) [Pubmed]
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