Single-strand breaks in double-stranded DNA irradiated in anoxic solution: contribution of tert-butanol radicals.
Yields of single-strand breaks induced by 60Co gamma or pulse irradiation in double-stranded calf thymus DNA have been measured in N2O-saturated aqueous solution as a function of the concentration of tert-butanol. The yields were found to be dependent on dose rate. The experimental data were analyzed using a theoretical model based on non-homogeneous scavenging kinetics. It is concluded from this analysis that after 60Co gamma irradiation in the absence of oxygen, aside from breaks caused by hydroxyl radicals, additional breaks occur which are initiated by hydrogen atoms and secondary radicals of tert-butanol. The efficiency of hydrogen atoms in causing single-strand breaks in double-stranded calf thymus DNA was determined to be 2.3%, while the rate constant for the reaction of tert-butanol radicals with DNA and their efficiency in causing single-strand breaks was determined to be 4.1 x 10(3) dm3 mol-1 s-1 and 2%, respectively.[1]References
- Single-strand breaks in double-stranded DNA irradiated in anoxic solution: contribution of tert-butanol radicals. Udovicić, L., Mark, F., Bothe, E. Radiat. Res. (1996) [Pubmed]
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