The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Expression of glyceraldehyde-3-phosphate dehydrogenase during differentiation of HD3 cells.

The chicken erythroblast cell line HD3, which is infected with a temperature-sensitive avian erythroleukemia virus, becomes committed to differentiate to an erythrocyte upon temperature shift in the presence of inducers (hemin and butyric acid). The activity of glyceraldehyde-3-phosphate dehydrogenase (GAD), a key enzyme in the glycolytic pathway, was examined. Upon induction of differentiation the following changes in glyceraldehyde-3-phosphate dehydrogenase activity and the corresponding mRNA level occurred. Twenty-four hours post-induction the glyceraldehyde-3-phosphate dehydrogenase message decreased and virtually disappeared within 48 h. Glyceraldehyde-3-phosphate dehydrogenase activity did not follow the mRNA level and increased within 48 h post-induction and then started to fall. The discrepancy between glyceraldehyde-3-phosphate dehydrogenase activity and the mRNA level is likely due to a difference in GAD protein and mRNA half-lives. The results also suggest that enzyme activity could be regulated by post-translational events. Chicken erythrocytes expressed reduced levels of glyceraldehyde-3-phosphate dehydrogenase activity. Thus the low level of GAD found in chicken erythrocytes is associated with a turn off of GAD gene expression upon induction of erythroid differentiation.[1]


WikiGenes - Universities