Solid-phase extraction and HPLC determination of levamisole hydrochloride in sheep plasma.
The anthelmintic, levamisole, was determined in sheep plasma by means of ion-pair solid-phase extraction (SPE) and reverse-phase liquid chromatography. The SPE columns were conditioned with 2 ml of methanol followed by 1 ml of octane sulphonic-acid buffer. After sample application, the columns were washed with 2 ml of the same buffer, followed by elution with 90/10 acetonitrile:buffer. A phenyl reverse-phase column (Spherisorb S5 Phenyl, 250 x 4.6 mm) was used with a mobile phase of acetonitrile: 0.005 M of octane sulphonic-acid sodium salt and 0.2% triethylamine in water, pH 3.5, 38/62. Extraction recoveries of 89-94% were achieved over the range from 100-3750 ng/ml. Accuracy and precision were better than 96% and 2.6%, respectively, over said range, with a limit of quantitation of 50 ng/ ml.[1]References
- Solid-phase extraction and HPLC determination of levamisole hydrochloride in sheep plasma. Du Preez, J.L., Lötter, A.P. Onderstepoort J. Vet. Res. (1996) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
