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Hackbarth, C.J. et al.

Hackbarth, Unsal, Chambers,

Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.

A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.[1]

References

Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra. Hackbarth, C.J., Unsal, I., Chambers, H.F. Antimicrob. Agents Chemother. (1997) [Pubmed]

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