Cloning and mutational analysis of the gene for the stationary-phase inducible catalase (catC) from Pseudomonas putida.
Pseudomonas putida, a bacterium that colonizes plant roots and enhances plant growth, produces three isozymes of catalase (A, B, and C) in stationary-phase cells. A catalase probe, generated by PCR analysis of P. putida genomic DNA with oligomers based on typical catalase sequences, hybridized to a genomic clone that expressed catalase C in Escherichia coli. The catC gene from this clone had a 2,133-bp open reading frame with a high level of identity to the stationary-phase-specific E. coli katE. Chromosomal mutants of P. putida deficient in catalase C, obtained by gene interruption with a luxAB-npt cassette, demonstrated enhanced catC transcription in stationary-phase cells and, upon exposure to phenol, in logarithmic-phase cells. The catalase C-deficient cells were not impaired in their ability to colonize roots of bean or wheat plants grown under sterile conditions.[1]References
- Cloning and mutational analysis of the gene for the stationary-phase inducible catalase (catC) from Pseudomonas putida. Miller, C.D., Kim, Y.C., Anderson, A.J. J. Bacteriol. (1997) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
