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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Jiao, K. et al.

Improved detection of polycyclic aromatic compounds in complex mixtures by liquid chromatographic fractionation on poly(divinylbenzene) prior to gas chromatography-mass spectrometry. Application to the analysis of diesel particulates.

Polycyclic aromatic compounds (PACs) are preferentially retained over other compound classes during high-performance liquid chromatography (HPLC) on poly(divinylbenzene) (PDVB) columns with a dichloromethane mobile phase. PAC retention during HPLC with PDVB/CH2Cl2 is governed by a multi-mode mechanism that has been previously described. This enhanced retention of PACs makes PDVB columns useful for isolating a PAC fraction from highly complex mixtures such as the emissions from fossil-fuels combustion. The cleaned-up PAC fraction yields a simple chromatogram with easily identified and quantified peaks without significant compound loss or danger of contamination. We illustrate the use of this clean-up method for the isolation of the PAC fraction from a standard reference diesel particulate sample.[1]

References

Improved detection of polycyclic aromatic compounds in complex mixtures by liquid chromatographic fractionation on poly(divinylbenzene) prior to gas chromatography-mass spectrometry. Application to the analysis of diesel particulates. Jiao, K., Lafleur, A.L. Journal of chromatography. A. (1997) [Pubmed]

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