Disease relevance of Rpa3

• Using a polyclonal antibody against recombinant p14, we show here that p14 is also localized to the nucleoli of murine mammary carcinomas and some human breast cancer samples [1].
• We have previously shown that the leader peptide (p14) of the Env-precursor of mouse mammary tumor virus is translocated into the nucleoli of murine T cell lymphomas that harbor this virus [1].

High impact information on Rpa3

• This function its essential for early embryogenesis and during tissue homeostasis, as revealed by epidermis-specific deletion of p14 [2].
• p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis [2].
• To gain insight into the role of BRCA2 in the repair of DNA damage, we fused a single (BRC3, BRC4) or multiple BRC motifs to the large RPA subunit [3].
• In an effort to identify cellular helicases that mimic the action of SV40 large T-antigen, we performed replication protein A (RPA) affinity chromatography on cell extracts from the mouse mammary carcinoma cell line FM3A [4].
• The properties of this enzyme suggest that it may take part in reactions mediated by RPA such as those predicted to occur at replication forks or alternatively may function during DNA repair or recombination [4].

Biological context of Rpa3

• CfaRPA1 contains a zinc-finger motif that is also found in the RP-A large subunits from human, frog, and yeast [5].
• The vector was modified to align the ribosome binding site with the p14 initiator codon, and transcription was placed under control of lambda cIts in a compatible plasmid [6].
• In vitro DNA binding assays demonstrated that SmCAR binds to the hsp27 ecdysone response element (EcRE) as well as schistosome p14 gene upstream region [7].
• Second, like the known potentiation of GABAergic synapses, the gamma2 down-regulation was present only after the onset of degeneration at p14 [8].
• Methylation-specific PCR targeting CpG-islands demonstrated markedly increased methylation of specific genes, such as p14, p15 and p16 genes, in EBVaGC in vivo [9].

Anatomical context of Rpa3

• The recombinant protein possessed an expected molecular mass of 14kDa and was located in bacterial inclusion bodies [10].

Analytical, diagnostic and therapeutic context of Rpa3

• A DNA helicase purified by replication protein A (RPA) affinity chromatography from mouse FM3A cells [4].
• Immunofluorescence experiments indicate that p14 accumulates in the cytoplasm of infected L cells [6].

References