Disease relevance of 4-Methylumbelliferylguanidinobenzoate

• 4-methylumbelliferylguanidinobenzoate reactive plasma "protease" in cystic fibrosis is albumin [1].

High impact information on 4-Methylumbelliferylguanidinobenzoate

• In contrast, 4-methylumbelliferylguanidinobenzoate-reactive protease activity in amniotic fluid from the 6 affected pregnancies was normal [2].
• Like plasma, the protease(s) hydrolyzed esters of arginine, were reactive towards 4-methylumbelliferylguanidinobenzoate (MUGB), a sensitive active site titrant of trypsin-like enzymes, and had a pI of 5.1--5 [3].
• Reaction of 4-methylumbelliferylguanidinobenzoate with proteases in human amniotic fluid [3].
• Reaction of 4-methylumbelliferylguanidinobenzoate with cultivated human skin fibroblasts derived from patients with cystic fibrosis [4].
• In contrast to the original reports by others, the hydrolysis of 4-methylumbelliferylguanidinobenzoate ((MUGB), and active-site titrant of serine proteases, was found not to be significantly different in plasma samples from patients with cystic fibrosis (CF), obligate heterozygotes, and normal controls [5].

Biological context of 4-Methylumbelliferylguanidinobenzoate

• Protease activity in cultivated human skin fibroblasts has been quantitated using 4-methylumbelliferylguanidinobenzoate (MUGB), an active site titrant of trypsin-like proteases (7) [4].
• Over 1,000 specimens of midtrimester human amniotic fluid have been analyzed for 4-methylumbelliferylguanidinobenzoate (MUGB) reactivity [6].
• Systematic study of the hydrolysis of 4-methylumbelliferylguanidinobenzoate in plasma from patients with cystic fibrosis and controls [5].

References