Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Chemical Compound Review:

AC1L9ARU 2-acetamido-N-[(2R,4R,5R)-5- (6...

Synonyms:

Lacalle, R.A. et al., Lacalle, R.A. et al., Tercero, J.A. et al.

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Disease relevance of N-Acetylpuromycin

In addition to pac and dmpM, two other genes have been identified in the pur cluster: pacHY, which determines an N-acetylpuromycin hydrolase and prg1, whose deduced amino acid sequence is significantly similar to that of degT, a Bacillus stearothermophilus pleiotropic regulatory gene [1].
Culture filtrates from either this organism or Streptomyces lividans transformants harboring the puromycin biosynthetic gene cluster cloned in low-copy-number cosmids contained an enzymic activity which hydrolyzes N-acetylpuromycin to produce the active antibiotic [2].

High impact information on N-Acetylpuromycin

Nine open reading frames are transcribed rightward as a unit in opposite direction to that of the pur8 gene which is expressed as a monocistronic transcript from the right-most end. napH encodes the known N-acetylpuromycin N-acetylhydrolase [3].

References

Cloning of the complete biosynthetic gene cluster for an aminonucleoside antibiotic, puromycin, and its regulated expression in heterologous hosts. Lacalle, R.A., Tercero, J.A., Jiménez, A. EMBO J. (1992) [Pubmed]
Identification of the gene encoding an N-acetylpuromycin N-acetylhydrolase in the puromycin biosynthetic gene cluster from Streptomyces alboniger. Lacalle, R.A., Tercero, J.A., Vara, J., Jimenez, A. J. Bacteriol. (1993) [Pubmed]
The biosynthetic pathway of the aminonucleoside antibiotic puromycin, as deduced from the molecular analysis of the pur cluster of Streptomyces alboniger. Tercero, J.A., Espinosa, J.C., Lacalle, R.A., Jiménez, A. J. Biol. Chem. (1996) [Pubmed]

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