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Gene Review:

bioB - biotin synthase

Escherichia coli CFT073

Sanyal, I. et al., Tamarit, J. et al., Tse Sum Bui, B. et al.

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Disease relevance of bioB

Biotin synthase catalyzes the chemically difficult final step in the biotin biosynthetic pathway and is encoded by the bioB gene in Escherichia coli [1].

High impact information on bioB

Such a sequence is also present in the activase of the pyruvate formate-lyase and in the biotin synthase, both carrying an iron-sulfur center involved in reductive activation of S-adenosylmethionine [2].
Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions [3].
However, 5'-deoxy-5'-methylthioadenosine, the postulated breakdown product from the action of 7,8-diaminopelargonic acid aminotransferase on Ado-Met, cannot be the active material since it has no stimulatory effect when added to the biotin synthase reaction mixture [1].
The mixture contains biotin synthase, flavodoxin, flavodoxin reductase, NADPH, Ado-Met, Fe, fructose-1,6-bisphosphate, cysteine, and dithiothreitol [1].

References

Escherichia coli biotin synthase: an investigation into the factors required for its activity and its sulfur donor. Sanyal, I., Gibson, K.J., Flint, D.H. Arch. Biochem. Biophys. (1996) [Pubmed]
The activating component of the anaerobic ribonucleotide reductase from Escherichia coli. An iron-sulfur center with only three cysteines. Tamarit, J., Gerez, C., Meier, C., Mulliez, E., Trautwein, A., Fontecave, M. J. Biol. Chem. (2000) [Pubmed]
Fate of the (2Fe-2S)(2+) cluster of Escherichia coli biotin synthase during reaction: a Mössbauer characterization. Tse Sum Bui, B., Benda, R., Schünemann, V., Florentin, D., Trautwein, A.X., Marquet, A. Biochemistry (2003) [Pubmed]

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