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Gene Review

bioB  -  biotin synthase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0764, JW0758
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Disease relevance of bioB


High impact information on bioB


Chemical compound and disease context of bioB


Biological context of bioB

  • A recombinant plasmid pSB01, containing an 8.2-kb DNA fragment from B. sphaericus, was isolated by phenotypic complementation of an Escherichia coli bioB strain [9].
  • Nucleotide sequence analysis of this fragment and N-terminal sequence determination of the recombinant protein product revealed that the bioB gene of B. sphaericus consists of a 996-bp open reading frame which is closely associated with at least one other gene [9].
  • Two other point mutations, which were both GC-->AT changes just before and after the initiation codon of the bioB gene, were considered to activate the translation efficiency [10].
  • These results suggest that this phenotype of growth inhibition by overexpression of the bioB gene in E. coli is independent of the biotin-forming activity itself, but is caused by some function involving a specific conformation of the bioB gene product [11].
  • This growth inhibition was still observed when the wild-type bioB gene was replaced by several mutant-type bioB genes derived from biotin auxotrophs that have base-pair substitutions creating amino acid substitutions in the bioB gene product [11].

Anatomical context of bioB


Associations of bioB with chemical compounds


Enzymatic interactions of bioB


Other interactions of bioB


Analytical, diagnostic and therapeutic context of bioB


  1. Genetic analysis of an incomplete bio operon in a biotin auxotrophic strain of Bacillus subtilis natto OK2. Sasaki, M., Kawamura, F., Kurusu, Y. Biosci. Biotechnol. Biochem. (2004) [Pubmed]
  2. Biotin synthase of Bacillus subtilis shows less reactivity than that of Escherichia coli in in vitro reaction systems. Kiyasu, T., Asakura, A., Nagahashi, Y., Hoshino, T. Arch. Microbiol. (2002) [Pubmed]
  3. Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli. Ollagnier-de Choudens, S., Sanakis, Y., Hewitson, K.S., Roach, P., Münck, E., Fontecave, M. J. Biol. Chem. (2002) [Pubmed]
  4. Identification of the [Fe-S] cluster-binding residues of Escherichia coli biotin synthase. McIver, L., Baxter, R.L., Campopiano, D.J. J. Biol. Chem. (2000) [Pubmed]
  5. The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon. Otsuka, A.J., Buoncristiani, M.R., Howard, P.K., Flamm, J., Johnson, C., Yamamoto, R., Uchida, K., Cook, C., Ruppert, J., Matsuzaki, J. J. Biol. Chem. (1988) [Pubmed]
  6. The iron-sulfur center of biotin synthase: site-directed mutants. Hewitson, K.S., Ollagnier-de Choudens, S., Sanakis, Y., Shaw, N.M., Baldwin, J.E., Münck, E., Roach, P.L., Fontecave, M. J. Biol. Inorg. Chem. (2002) [Pubmed]
  7. Structure-function studies of Escherichia coli biotin synthase via a chemical modification and site-directed mutagenesis approach. Farh, L., Hwang, S.Y., Steinrauf, L., Chiang, H.J., Shiuan, D. J. Biochem. (2001) [Pubmed]
  8. Escherichia coli biotin synthase produces selenobiotin. Further evidence of the involvement of the [2Fe-2S]2+ cluster in the sulfur insertion step. Tse Sum Bui, B., Mattioli, T.A., Florentin, D., Bolbach, G., Marquet, A. Biochemistry (2006) [Pubmed]
  9. Cloning of the biotin synthetase gene from Bacillus sphaericus and expression in Escherichia coli and Bacilli. Ohsawa, I., Speck, D., Kisou, T., Hayakawa, K., Zinsius, M., Gloeckler, R., Lemoine, Y., Kamogawa, K. Gene (1989) [Pubmed]
  10. Sequencing analysis of mutation points in the biotin operon of biotin-overproducing Escherichia coli mutants. Ifuku, O., Haze, S., Kishimoto, J., Koga, N., Yanagi, M., Fukushima, S. Biosci. Biotechnol. Biochem. (1993) [Pubmed]
  11. Molecular analysis of growth inhibition caused by overexpression of the biotin operon in Escherichia coli. Ifuku, O., Koga, N., Haze, S., Kishimoto, J., Arai, T., Wachi, Y. Biosci. Biotechnol. Biochem. (1995) [Pubmed]
  12. Biochemical characterization of the Arabidopsis biotin synthase reaction. The importance of mitochondria in biotin synthesis. Picciocchi, A., Douce, R., Alban, C. Plant Physiol. (2001) [Pubmed]
  13. The [4Fe-4S](2+) cluster in reconstituted biotin synthase binds S-adenosyl-L-methionine. Cosper, M.M., Jameson, G.N., Davydov, R., Eidsness, M.K., Hoffman, B.M., Huynh, B.H., Johnson, M.K. J. Am. Chem. Soc. (2002) [Pubmed]
  14. Biotin synthase is catalytic in vivo, but catalysis engenders destruction of the protein. Choi-Rhee, E., Cronan, J.E. Chem. Biol. (2005) [Pubmed]
  15. Biotin synthase: enzyme or reactant? Jarrett, J.T. Chem. Biol. (2005) [Pubmed]
  16. Biosynthesis of biotin and lipoic acid. Marquet, A., Bui, B.T., Florentin, D. Vitam. Horm. (2001) [Pubmed]
  17. Biotin synthesis in higher plants: purification and characterization of bioB gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells. Baldet, P., Alban, C., Douce, R. FEBS Lett. (1997) [Pubmed]
  18. The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase. Zhang, S., Sanyal, I., Bulboaca, G.H., Rich, A., Flint, D.H. Arch. Biochem. Biophys. (1994) [Pubmed]
  19. Evidence from Mössbauer spectroscopy for distinct [2Fe-2S](2+) and [4Fe-4S](2+) cluster binding sites in biotin synthase from Escherichia coli. Ugulava, N.B., Surerus, K.K., Jarrett, J.T. J. Am. Chem. Soc. (2002) [Pubmed]
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