Disease relevance of argH

• The mutation causing the altered electrophoretic mobility is located between argH and rif (79 min on the E. coli genetic map) [1].
• When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously acquired sensitivity to colicin E3 and phage BF23 [2].

High impact information on argH

• In the reciprocal experiment introducing bfe into an argH bfe+ recipient, it was found that colicin E3-resistant, arg+ cells began to appear shortly after the arg+ recombinant population began to divide [2].
• The ratios of steady-state argE and argH enzyme synthesis without arginine to that with arginine were 12 and 20, respectively, whereas the similar ratio for argECBH mRNA was 2 to 3 [3].
• In a comparative cross employing a haploid E. coli Hfr, in which rha inheritance was similar at 56%, argH inheritance was 41% [4].
• By inserting a T7 bacteriophage promoter into the plasmid, a version of the cDNA which is able to complement both the C. reinhardtii arg7 mutant and the Escherichia coli argH mutant has been created [5].
• The argH open reading frame encodes a protein comprised of 461 amino acids with a calculated molecular mass of 51,349 Da [6].

Biological context of argH

• Attempts to create an argH auxotroph of M. maripaludis by disrupting the genomic allele were unsuccessful: although a knockout allele of argH was integrated into the M. maripaludis chromosome by homologous recombination, the intact copy was not excluded, suggesting that the argH gene is essential [7].

Associations of argH with chemical compounds

• Genes argF and argH were not linked to other arginine biosynthetic genes by cross-complementation analysis [8].

References