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Gene Review

argF  -  ornithine carbamoyltransferase 2, chain F;...

Escherichia coli str. K-12 substr. MG1655

Synonyms: Arg5, ECK0274, JW0266, argD
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Disease relevance of argF

  • Under these conditions for hybridization, the gonococcal clones carrying argB or argF genes did not hybridize with plasmids containing the corresponding E. coli gene [1].
  • In Xanthomonas campestris, the protein annotated as ornithine transcarbamylase, and encoded by the argF gene, is unable to synthesize citrulline directly from ornithine [2].

High impact information on argF

  • The complete nucleotide sequence of argF is presented, together with that of an operator-constitutive mutant [3].
  • Mutation of Arg3 or Ile47 to alanine reduces binding affinity 10-20-fold while mutation of Arg5, Asn51, or Lys50 to alanine reduces binding affinity > 100-fold, indicating that both minor-groove and major-groove interactions contribute to the overall binding energy [4].
  • However, the interactions between Lys50 of the recognition helix and the major-groove edges of base pairs 5 and 6 are more specific than interactions mediated by Arg3 and Arg5 in the N-terminal arm and the minor-groove edges of base pairs 1 and 2 [4].
  • The plasmid was packaged in vivo into lambda phage particles, mutagenized in vitro with hydroxylamine, transduced into Escherichia coli, and mobilized to an argF auxotroph of P. aeruginosa FRD [5].
  • For this, a DNA fragment containing the alginate biosynthetic operon and adjacent argF gene in a gene replacement cosmid vector was utilized [5].

Biological context of argF


Associations of argF with chemical compounds

  • Two copies of IS1 which flank a chromosomal segment containing the argF gene (IS1B and IS1C) have identical nt sequences [7].
  • Upstream from argF is a putative gene for diphthine synthetase, a eukaryotic enzyme assumed to occur also in archaea but not in bacteria [8].
  • The minimum inhibitory concentration of kanamycin determined in the B. subtilis argC-neo transcriptional fusion pUL730 and expression of the argF gene product, ornithine carbamoyltransferase (OCTase), in pUL800 were reduced by approximately 3 and 2 fold respectively under conditions of arginine excess and in the presence of pUL2030 [9].

Other interactions of argF

  • The E. coli argA and argF enzymes, which are controlled by repression in their native host, were synthesized constitutively in P. aeruginosa at 5% of the levels measured in E. coli under derepressed conditions [10].


  1. Cloning and organization of seven arginine biosynthesis genes from Neisseria gonorrhoeae. Picard, F.J., Dillon, J.R. J. Bacteriol. (1989) [Pubmed]
  2. Acetylornithine transcarbamylase: a novel enzyme in arginine biosynthesis. Morizono, H., Cabrera-Luque, J., Shi, D., Gallegos, R., Yamaguchi, S., Yu, X., Allewell, N.M., Malamy, M.H., Tuchman, M. J. Bacteriol. (2006) [Pubmed]
  3. Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases--complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB. Van Vliet, F., Cunin, R., Jacobs, A., Piette, J., Gigot, D., Lauwereys, M., Piérard, A., Glansdorff, N. Nucleic Acids Res. (1984) [Pubmed]
  4. Specificity of minor-groove and major-groove interactions in a homeodomain-DNA complex. Ades, S.E., Sauer, R.T. Biochemistry (1995) [Pubmed]
  5. Identification of algF in the alginate biosynthetic gene cluster of Pseudomonas aeruginosa which is required for alginate acetylation. Franklin, M.J., Ohman, D.E. J. Bacteriol. (1993) [Pubmed]
  6. Purification and characterization of recombinant human granulocyte colony-stimulating factor (rhG-CSF) derivatives: KW-2228 and other derivatives. Yamasaki, M., Konishi, N., Yamaguchi, K., Itoh, S., Yokoo, Y. Biosci. Biotechnol. Biochem. (1998) [Pubmed]
  7. Four types of IS1 with differences in nucleotide sequence reside in the Escherichia coli K-12 chromosome. Umeda, M., Ohtsubo, E. Gene (1991) [Pubmed]
  8. Isolation of the gene encoding Pyrococcus furiosus ornithine carbamoyltransferase and study of its expression profile in vivo and in vitro. Roovers, M., Hethke, C., Legrain, C., Thomm, M., Glansdorff, N. Eur. J. Biochem. (1997) [Pubmed]
  9. Cloning in Escherichia coli of a Bacillus subtilis arginine repressor gene through its ability to confer structural stability on a fragment carrying genes of arginine biosynthesis. Smith, M.C., Mountain, A., Baumberg, S. Mol. Gen. Genet. (1986) [Pubmed]
  10. Expression of biosynthetic genes from Pseudomonas aeruginosa and Escherichia coli in the heterologous host. Jeenes, D.J., Soldati, L., Baur, H., Watson, J.M., Mercenier, A., Reimmann, C., Leisinger, T., Haas, D. Mol. Gen. Genet. (1986) [Pubmed]
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