The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

ISRm3  -  pseudo

Sinorhizobium meliloti 1021

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ISRm3


High impact information on ISRm3

  • An open reading frame that spans 89% of the length of one DNA strand encodes a putative transposase with significant similarity to the putative transposases of 11 insertion sequence elements from diverse bacterial species, including ISRm3 from R. meliloti [5].
  • Multiple copies and variants of ISRm5 occur in the R. meliloti genome, often in close association with ISRm3 [5].
  • Nucleotide sequence of Rhizobium meliloti GR4 insertion sequence ISRm3 linked to the nodulation competitiveness locus nfe [6].
  • Upon examination of the extended DNA region containing host-specific nodulation genes nodFEG and nodH, we found that the nodG gene of WL131 bears a novel insertion sequence, ISRm3 [7].
  • Analysis of six copies of ISRm3 in two strains showed that a variable number of base pairs (usually eight or nine) were duplicated and formed direct repeats adjacent to the site of insertion [2].

Biological context of ISRm3

  • The entire nucleotide sequence of ISRm3 in R. meliloti 102F70 is 1,298 bp and has 30-bp terminal inverted repeats which are perfectly matched [2].
  • This plasmid is indigenous to strain IZ450 and fortuitously contained four copies of ISRm3 [2].

Analytical, diagnostic and therapeutic context of ISRm3

  • Genomic DNA fingerprinting with insertion elements ISRm3 and ISRm2011-2 suggested that transposition of these elements was not a cause of variation, but a DNA band was absent in the profiles of two out of three W2-like colonies [8].


  1. Isolation and analysis of IS6120, a new insertion sequence from Mycobacterium smegmatis. Guilhot, C., Gicquel, B., Davies, J., Martín, C. Mol. Microbiol. (1992) [Pubmed]
  2. Identification and nucleotide sequence of Rhizobium meliloti insertion sequence ISRm3: similarity between the putative transposase encoded by ISRm3 and those encoded by Staphylococcus aureus IS256 and Thiobacillus ferrooxidans IST2. Wheatcroft, R., Laberge, S. J. Bacteriol. (1991) [Pubmed]
  3. Identification and activity of two insertion sequence elements in Rhodococcus sp. strain IGTS8. Denome, S.A., Young, K.D. Gene (1995) [Pubmed]
  4. Characterization of IS1201, an insertion sequence isolated from Lactobacillus helveticus. Tailliez, P., Ehrlich, S.D., Chopin, M.C. Gene (1994) [Pubmed]
  5. Characterization, nucleotide sequence, and conserved genomic locations of insertion sequence ISRm5 in Rhizobium meliloti. Laberge, S., Middleton, A.T., Wheatcroft, R. J. Bacteriol. (1995) [Pubmed]
  6. Nucleotide sequence of Rhizobium meliloti GR4 insertion sequence ISRm3 linked to the nodulation competitiveness locus nfe. Soto, M.J., Zorzano, A., Olivares, J., Toro, N. Plant Mol. Biol. (1992) [Pubmed]
  7. Analysis of Rhizobium meliloti nodulation mutant WL131: novel insertion sequence ISRm3 in nodG and altered nodH protein product. Ogawa, J., Brierley, H.L., Long, S.R. J. Bacteriol. (1991) [Pubmed]
  8. Colony variation in Sinorhizobium meliloti inoculant strain U 45. Bloem, J.F., Botha, W.J., Law, I.J., Steyn, P.L. Microbiol. Res. (2002) [Pubmed]
WikiGenes - Universities