Disease relevance of pyrF

• Translational coupling in the pyrF operon of Salmonella typhimurium [1].

High impact information on pyrF

• Locus tppB maps at 27 min in the cotransduction gap between purB and pyrF [2].
• (i) The expression of pyrB, pyrE, and pyrF is controlled by a uridine nucleotide in a noncoordinate manner [3].
• The pyrF gene, encoding the sixth enzyme of pyrimidine biosynthesis in Salmonella typhirmurium, appears to be the first gene of an operon [1].
• Inspection of the nucleotide sequence of the pyrF/orfF junction region suggests that formation of secondary structures on the naked mRNA may explain the low level of orfF expression in the absence of translation of the pyrF terminal region [1].
• This was investigated by studying how frameshift mutations, which cause premature termination of pyrF translation at different points, affect orfF expression [1].

Biological context of pyrF

• Furthermore, it was found that the pyrF gene is expressed as the first gene of a bicistronic operon, wherein the second gene encodes an 11-kDa polypeptide of unknown functions [4].
• Another open reading frame, with a translational start codon which overlaps the translational stop codons of the pyrF gene, encodes a polypeptide of 11513 Da [4].
• The complete nucleotide sequence of the pyrF operon was determined [4].

Associations of pyrF with chemical compounds

• Moreover, aspartate transcarbamylase (encoded by pyrB) became further derepressed upon cytidine addition, whereas no change occurred in the levels of the last two enzymes (encoded by pyrE and pyrF) of the pyrimidine pathway [5].
• Aspartate carbamoyltransferase showed the greatest degree of derepression of the six enzymes studied, with pyrA strains (blocked in the first step of the pathway) showing about twice as much derepression as pyrF strains (blocked in the sixth step of the pathway) [6].

References