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Gene Review

pyrE  -  orotate phosphoribosyltransferase

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

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Disease relevance of pyrE

  • RNA polymerase involvement in the regulation of expression of Salmonella typhimurium pyr genes. Isolation and characterization of a fluorouracil-resistant mutant with high, constitutive expression of the pyrB and pyrE genes due to a mutation in rpoBC [1].

High impact information on pyrE


Chemical compound and disease context of pyrE


Biological context of pyrE

  • Together with the phenotype of the rpoBC mutant, these results indicate that expression of pyrB and pyrE is regulated by the mRNA chain growth rate, which is controlled by the cellular UTP pool [6].
  • We investigated the transcription kinetics of RNA polymerase from an rpoBC mutant of Salmonella typhimurium which showed highly elevated, constitutive expression of the pyrB and pyrE genes as well as an increased cellular pool of UTP [6].
  • The leader region does not show any features resembling the attenuators found preceding the pyrBI operon and the pyrE gene [7].
  • The mutation was found to be a single base-pair transition of GC to AT, and occurred within a region of dyad symmetry (attenuator) located just upstream of the pyrE structural gene [8].

Associations of pyrE with chemical compounds

  • Moreover, aspartate transcarbamylase (encoded by pyrB) became further derepressed upon cytidine addition, whereas no change occurred in the levels of the last two enzymes (encoded by pyrE and pyrF) of the pyrimidine pathway [9].
  • (i) The expression of pyrB, pyrE, and pyrF is controlled by a uridine nucleotide in a noncoordinate manner [10].
  • However, the affected genes of several such 'FOR' (FO-resistant) mutants were shown by transduction of map in the short cysE-pyrE segment, which includes nearly all known rfa genes responsible for synthesis of LPS core [11].
  • Orotate phosphoribosyltransferase (OMP synthase, EC forms the UMP precursor orotidine 5'-monophophate (OMP) from orotate and alpha-D-5-phosphoribosyl-1-pyrophosphate (PRPP) [12].
  • There are five highly conserved lysine residues (Lys-19, -26, -73, -100, and -103) in S. typhimurium OPRTase [4].

Other interactions of pyrE


  1. RNA polymerase involvement in the regulation of expression of Salmonella typhimurium pyr genes. Isolation and characterization of a fluorouracil-resistant mutant with high, constitutive expression of the pyrB and pyrE genes due to a mutation in rpoBC. Jensen, K.F., Neuhard, J., Schack, L. EMBO J. (1982) [Pubmed]
  2. Active site lysines in orotate phosphoribosyltransferase. Grubmeyer, C., Segura, E., Dorfman, R. J. Biol. Chem. (1993) [Pubmed]
  3. Motional dynamics of the catalytic loop in OMP synthase. Wang, G.P., Cahill, S.M., Liu, X., Girvin, M.E., Grubmeyer, C. Biochemistry (1999) [Pubmed]
  4. Locations and functional roles of conserved lysine residues in Salmonella typhimurium orotate phosphoribosyltransferase. Ozturk, D.H., Dorfman, R.H., Scapin, G., Sacchettini, J.C., Grubmeyer, C. Biochemistry (1995) [Pubmed]
  5. Crystal structure of orotate phosphoribosyltransferase. Scapin, G., Grubmeyer, C., Sacchettini, J.C. Biochemistry (1994) [Pubmed]
  6. Association of RNA polymerase having increased Km for ATP and UTP with hyperexpression of the pyrB and pyrE genes of Salmonella typhimurium. Jensen, K.F., Fast, R., Karlström, O., Larsen, J.N. J. Bacteriol. (1986) [Pubmed]
  7. Cloning and characterization of the pyrF operon of Salmonella typhimurium. Theisen, M., Kelln, R.A., Neuhard, J. Eur. J. Biochem. (1987) [Pubmed]
  8. A chromosomal mutation mediating increased expression of pyrE in Salmonella typhimurium is located within the proposed attenuator. Neuhard, J., Kelln, R.A. Can. J. Microbiol. (1988) [Pubmed]
  9. Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine. Kelln, R.A., Kinahan, J.J., Foltermann, K.F., O'Donovan, G.A. J. Bacteriol. (1975) [Pubmed]
  10. Control of expression of the pyr genes in Salmonella typhimurium: effects of variations in uridine and cytidine nucleotide pools. Schwartz, M., Neuhard, J. J. Bacteriol. (1975) [Pubmed]
  11. Mutations in Salmonella typhimurium conferring resistance to Felix O phage without loss of smooth character. MacPhee, D.G., Krishnapillai, V., Roantree, R.J., Stocker, B.A. J. Gen. Microbiol. (1975) [Pubmed]
  12. Kinetic mechanism of OMP synthase: a slow physical step following group transfer limits catalytic rate. Wang, G.P., Lundegaard, C., Jensen, K.F., Grubmeyer, C. Biochemistry (1999) [Pubmed]
  13. Mutations in the spoT gene of Salmonella typhimurium: effects on his operon expression. Rudd, K.E., Bochner, B.R., Cashel, M., Roth, J.R. J. Bacteriol. (1985) [Pubmed]
  14. Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase. Köstner, M., Schmidt, B., Bertram, R., Hillen, W. Appl. Environ. Microbiol. (2006) [Pubmed]
  15. Altered linkage values in phage P22--mediated transduction caused by distant deletions or insertions in donor chromosomes. Krajewska-Grynkiewicz, K., Kłopotowski, T. Mol. Gen. Genet. (1979) [Pubmed]
  16. Enzymatic coupled assay procedures that employ high-performance liquid chromatography: the synthesis of orotidylate from ribose. Ashton, R.W., Sloan, D.L. J. Chromatogr. (1984) [Pubmed]
  17. Repression and derepression of the enzymes of the pyrimidine biosynthetic pathway in Salmonella typhimurium. Smith, J.M., Kelln, R.A., O'Donovan, G.A. J. Gen. Microbiol. (1980) [Pubmed]
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