Disease relevance of hycG

• Based on available annotated gene sequence information, the enteric pathogen salmonella, like other enteric bacteria, contains three putative membrane-associated H2-using hydrogenase enzymes [1].
• Characterization and physiological roles of membrane-bound hydrogenase isoenzymes from Salmonella typhimurium [2].
• The S. typhimurium enzymes cross-reacted with antibodies raised to the respective hydrogenase isoenzymes of E. coli [2].

High impact information on hycG

• The introduction of one of the hydrogenase genes into the triple mutant strain on a low-copy-number plasmid resulted in a strain that was able to both oxidize H2 and cause morbidity in mice within 11 days of inoculation; therefore, the avirulent phenotype of the triple mutant is not due to an unknown spurious mutation [1].
• Two acid-inducible genes, aniC and aciK, that require anaerobiosis and tyrosine for expression were identified as orf326a encoding a potential amino acid/polyamine antiporter and hyaB encoding hydrogenase I, respectively [3].
• Mutations in oxrC were highly pleiotropic, preventing the anaerobic expression of the formate dehydrogenase component of formate hydrogen lyase (fhl), a tripeptidase (pepT), and two of the three known hydrogenase isoenzymes (hydrogenases 1 and 3) [4].
• The synthesis of the individual hydrogenase isoenzymes is also specifically influenced by fnr and oxrC mutations and by catabolite repression in a manner entirely consistent with the proposed role for each isoenzyme in hydrogen metabolism [5].
• Hydrogenase 3 activity correlated closely with formate hydrogenlyase-dependent hydrogen evolution, whereas isoenzyme 2 catalyzed hydrogen uptake (oxidation) during anaerobic, respiration-dependent growth [2].

Biological context of hycG

• Since the stn gene was located opposite the hydrogenase regulatory genes (hydHG) required for hydrogen metabolism in bacteria, our data suggested that only in Salmonella and some other members of the family Enterobacteriaceae had the DNA sequence evolved, presumably through point mutations, into an expressed gene product of similar size [6].

Associations of hycG with chemical compounds

• A second group consisted of mutants which lacked the formate dehyrogenase associated with hydrogenase [7].
• 3. A new scheme for the formate hydrogenylase system is proposed, in which hydrogenase transfers electrons directly to benzyl viologen [8].

Other interactions of hycG

• Unlike the expression of hydrogenases 2 and 3, hydrogenase 1 expression was both fnr and oxrC dependent [5].

Analytical, diagnostic and therapeutic context of hycG

• The hydBGDA genes, which encode the four subunits beta, gamma, delta and alpha of the [Ni-Fe] hydrogenase from the archaeon Pyrococcus furiosus, have been isolated and sequenced using a PCR/IPCR-based strategy [9].
• Cloning and sequence analysis of hydrogenase regulatory genes (hydHG) from Salmonella typhimurium [10].

References