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Gene Review:

traA - conjugal transfer pilin subunit TraA

Escherichia coli

Koraimann, G. et al., Frost, L.S. et al., Finlay, B.B. et al., Zahrl, D. et al., Finlay, B.B. et al.

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High impact information on traA

A traA null mutant of plasmid R1-16, which lacks the functional gene encoding the major pilus protein pilin, showed distinctly reduced stress responses [1].
The pED208 traA and traL genes were separated by a single base pair, and no ribosome binding site preceded the traL gene [2].
The traA reading frame has been located both genetically and by comparing the primary structure of F pilin (the traA product) predicted by the DNA sequence to the amino acid composition and sequence of N- and C-terminal peptides isolated from purified F pilin [3].
A chimera containing the ColB2 pilin gene was able to complement an F traA mutant, demonstrating that the pilus assembly proteins of F can utilize the ColB2 pilin protein to form a pilus [4].
Processing of the transfer operon mRNA of the conjugative resistance plasmid R1-19 results in the accumulation of stable traA mRNAs [5].

Biological context of traA

Half-life determinations reveal the same half-lives of 13 +/- 2 min for the traA mRNAs transcribed from hybrid lac-traAL-cat test plasmids, the R1-19 plasmid, and the F plasmid [5].

References

Expression and assembly of a functional type IV secretion system elicit extracytoplasmic and cytoplasmic stress responses in Escherichia coli. Zahrl, D., Wagner, M., Bischof, K., Koraimann, G. J. Bacteriol. (2006) [Pubmed]
Nucleotide sequence of the tra YALE region from IncFV plasmid pED208. Finlay, B.B., Frost, L.S., Paranchych, W. J. Bacteriol. (1986) [Pubmed]
DNA sequence of the F traALE region that includes the gene for F pilin. Frost, L.S., Paranchych, W., Willetts, N.S. J. Bacteriol. (1984) [Pubmed]
Localization, cloning, and sequence determination of the conjugative plasmid ColB2 pilin gene. Finlay, B.B., Frost, L.S., Paranchych, W. J. Bacteriol. (1984) [Pubmed]
Differential mRNA decay within the transfer operon of plasmid R1: identification and analysis of an intracistronic mRNA stabilizer. Koraimann, G., Teferle, K., Mitteregger, R., Wagner, S., Högenauer, G. Mol. Gen. Genet. (1996) [Pubmed]

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