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Gene Review:

traD - DNA transport

Escherichia coli

Jalajakumari, M.B. et al., Jalajakumari, M.B. et al., Maneewannakul, K. et al., Barany, F. et al., Panicker, M.M. et al., et al.

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Disease relevance of traD

Nucleotide sequence of the traD region in the Escherichia coli F sex factor [1].
They were relatively insensitive to bacteriophage R17, as expected from the absence of traD [2].
DNA transport and integration in Haemophilus influenzae transformation was studied with a plasmid clone of homologous DNA (pCML6) [3].

High impact information on traD

Using a traD overexpression plasmid, we purified the F sex factor TraD protein in milligram quantities [4].
Construction of derivatives of the F plasmid pOX-tra715: characterization of traY and traD mutants that can be complemented in trans [5].
An open reading frame, preceded by a typical promoter sequence and a weak and poorly located Shine-Dalgarno sequence, follows traT and corresponds to the start of traD [6].
A previously unidentified open reading frame (tentatively trbH) lies between traD and traI [7].
An open reading frame (ORF) of 2148 bp within the sequence confirms that traD encodes an 81.4-kDa cytoplasmic membrane protein [1].

References

Nucleotide sequence of the traD region in the Escherichia coli F sex factor. Jalajakumari, M.B., Manning, P.A. Gene (1989) [Pubmed]
Structure and function of conjugative pili: inducible synthesis of functional F pili by Escherichia coli K-12 containing a lac-tra operon fusion. Grossman, T.H., Silverman, P.M. J. Bacteriol. (1989) [Pubmed]
Directional transport and integration of donor DNA in Haemophilus influenzae transformation. Barany, F., Kahn, M.E., Smith, H.O. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
Purification and properties of the F sex factor TraD protein, an inner membrane conjugal transfer protein. Panicker, M.M., Minkley, E.G. J. Biol. Chem. (1992) [Pubmed]
Construction of derivatives of the F plasmid pOX-tra715: characterization of traY and traD mutants that can be complemented in trans. Maneewannakul, K., Kathir, P., Endley, S., Moore, D., Manchak, J., Frost, L., Ippen-Ihler, K. Mol. Microbiol. (1996) [Pubmed]
Surface exclusion genes traS and traT of the F sex factor of Escherichia coli K-12. Determination of the nucleotide sequence and promoter and terminator activities. Jalajakumari, M.B., Guidolin, A., Buhk, H.J., Manning, P.A., Ham, L.M., Hodgson, A.L., Cheah, K.C., Skurray, R.A. J. Mol. Biol. (1987) [Pubmed]
Nucleotide sequence of the traI (helicase I) gene from the sex factor F. Bradshaw, H.D., Traxler, B.A., Minkley, E.G., Nester, E.W., Gordon, M.P. J. Bacteriol. (1990) [Pubmed]

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