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Gene Review:

orf17 - hypothetical protein

Escherichia coli

O'Handley, S.F. et al., Méjean, V. et al., Hori, M. et al., Mohan, S. et al., Arulanantham, H. et al.

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Disease relevance of orf17

The shorter version of orf17 was successfully expressed in Escherichia coli and purified as a monomeric protein [1].

High impact information on orf17

The product of the Escherichia coli orf17 gene is a novel nucleoside triphosphate pyrophosphohydrolase with a preference for dATP over the other canonical (deoxy)nucleoside triphosphates, and it catalyzes the hydrolysis of dATP through a nucleophilic attack at the beta-phosphorus to produce dAMP and inorganic pyrophosphate [2].
We suggest that bypass of the lpxA2 phenotype by mutations in orf17 may be due to an increased (3R)-hydroxymyristoyl-ACP pool [3].
Computer searching for sequence homology to MutX uncovered a second E. coli protein, the product of orf17, a gene of unknown function located near the ruvC gene [4].
To examine its in vivo role(s) in bacteria, plasmid DNAs containing the orf17 gene in the sense and antisense orientations were introduced [5].

References

ORF17 from the clavulanic acid biosynthesis gene cluster catalyzes the ATP-dependent formation of N-glycyl-clavaminic acid. Arulanantham, H., Kershaw, N.J., Hewitson, K.S., Hughes, C.E., Thirkettle, J.E., Schofield, C.J. J. Biol. Chem. (2006) [Pubmed]
Escherichia coli orf17 codes for a nucleoside triphosphate pyrophosphohydrolase member of the MutT family of proteins. Cloning, purification, and characterization of the enzyme. O'Handley, S.F., Frick, D.N., Bullions, L.C., Mildvan, A.S., Bessman, M.J. J. Biol. Chem. (1996) [Pubmed]
An Escherichia coli gene (FabZ) encoding (3R)-hydroxymyristoyl acyl carrier protein dehydrase. Relation to fabA and suppression of mutations in lipid A biosynthesis. Mohan, S., Kelly, T.M., Eveland, S.S., Raetz, C.R., Anderson, M.S. J. Biol. Chem. (1994) [Pubmed]
Characterization of the mutX gene of Streptococcus pneumoniae as a homologue of Escherichia coli mutT, and tentative definition of a catalytic domain of the dGTP pyrophosphohydrolases. Méjean, V., Salles, C., Bullions, L.C., Bessman, M.J., Claverys, J.P. Mol. Microbiol. (1994) [Pubmed]
Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme. Hori, M., Asanuma, T., Inanami, O., Kuwabara, M., Harashima, H., Kamiya, H. Biol. Pharm. Bull. (2006) [Pubmed]

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