Disease relevance of ruvC

• The results of these experiments indicated that the lambda rap gene could functionally substitute for the E. coli ruvC gene in Red-mediated recombination [1].
• The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis [2].

High impact information on ruvC

• We propose that efficient RuvC resolution requires the translocation of Holliday junctions to specific cleavage sites, thus providing a biochemical basis for the similar genetic defects observed in ruvA, ruvB, and ruvC mutants [3].
• Partial suppression of the fission yeast rqh1(-) phenotype by expression of a bacterial Holliday junction resolvase [4].
• Here, we used a combination of two-dimensional gel analysis and electron microscopy to reveal that although ruvAB and ruvC mutants are able to resume replication following arrest at UV-induced lesions, molecules that replicate in the presence of DNA damage accumulate unresolved Holliday junctions [5].
• Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions [6].
• Indeed, (i) priA recB mutant chromosomes are linearized by a reaction that requires the presence of the Holliday junction resolvase RuvABC, and (ii) RuvABC-dependent linearization is prevented by the presence of RecBC [7].

Biological context of ruvC

• Multicopy plasmids expressing RusA alone make ruvA, ruvB, and ruvC mutants resistant to UV light [8].
• The product of the ruvC gene, which is not regulated by the SOS system, resolves Holiday junctions by introducing nicks at or near the crossover junction in strands with the same polarity at the same sites [9].
• To probe the validity of the E. coli paradigm, we examined the impact of mutations in DeltaruvAB and DeltarecU (a ruvC functional analog) on DNA repair [2].
• Two further open reading frames of unknown function were identified, one on either side of the orf-33-ruvC operon [10].
• The ruvC gene of Escherichia coli specifies a nuclease that resolves Holliday junction intermediates in genetic recombination (B. Connolly, C.A. Parsons, F.E. Benson, H.J. Dunderdale, G.J. Sharples, R.G. Lloyd, and S.C. West, Proc. Natl. Acad, Sci. USA 88:6063-6067, 1991) [10].

Other interactions of ruvC

• Mutations in recB, required for double-strand break repair, and in ruvC, the Holliday junction resolvase gene, were synthetically lethal with dnaB107, causing enhanced temperature sensitivity [11].
• In Escherichia coli, the ruvA, ruvB and ruvC gene products are required for genetic recombination and the recombinational repair of DNA damage [12].

Analytical, diagnostic and therapeutic context of ruvC

• Crystallization of RusA Holliday junction resolvase from Escherichia coli [13].

References