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Gene Review:

39k - PP31; AcMNPV orf36

Bombyx mori nucleopolyhedrovirus

Mistretta, T.A. et al., Gong, M. et al., Choi, J. et al., Xu, B. et al., Yoo, S. et al., et al.

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Disease relevance of 39k

The addition of purified protein to transcription assays containing baculovirus RNA polymerase stimulated transcription of the very late polyhedrin promoter but not the late 39k promoter [1].

High impact information on 39k

Transcription from the very late polyhedrin promoter was fivefold higher than that from the 39k late promoter with the nuclear extract prepared at 36 h postinfection [2].
These data suggested that ORF-121 stimulated 39k expression by upregulation of IE1 expression [3].
A titration experiment was done to determine the minimal amounts of IE1 required for activation of the 39k promoter in the presence and absence of a cis-linked enhancer element [4].
One of these, ie2 (formerly called IEN), was mapped and identified by its ability to augment IE1-mediated transactivation of the delayed early 39k gene [5].
To identify additional viral proteins that regulate expression from the 39k promoter, we performed cotransfection experiments with clones of viral DNA and a plasmid containing the chloramphenicol acetyltransferase (CAT) gene under the control of the 39k promoter [6].

References

Transcriptional activity of baculovirus very late factor 1. Mistretta, T.A., Guarino, L.A. J. Virol. (2005) [Pubmed]
Differential transcription of baculovirus late and very late promoters: fractionation of nuclear extracts by phosphocellulose chromatography. Xu, B., Yoo, S., Guarino, L.A. J. Virol. (1995) [Pubmed]
Mapping of ORF121, a factor that activates baculovirus early gene expression. Gong, M., Jin, J., Guarino, L.A. Virology (1998) [Pubmed]
Expression of the IE1 transactivator of Autographa californica nuclear polyhedrosis virus during viral infection. Choi, J., Guarino, L.A. Virology (1995) [Pubmed]
The Autographa californica nuclear polyhedrosis virus ie2 gene encodes a transcriptional regulator. Yoo, S., Guarino, L.A. Virology (1994) [Pubmed]
Expression of the 39k promoter of Autographa californica nuclear polyhedrosis virus is increased by the apoptotic suppressor P35. Gong, M., Guarino, L.A. Virology (1994) [Pubmed]

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