High impact information on Nup62

• Immunogold electron microscopy and in vitro import assays showed that the active N-62 mutant was not imported into the mitochondria [1].
• First, a fluorescent NBD-cholesterol binding assay revealed 2 sterol binding sites (K(d) values near 32 nm), whereas the inactive A218V N-62 StAR mutant had only a single binding site with 8-fold lower affinity [2].
• However, it has not been shown how the interaction of Nup62/p62 with transport factors is involved in nucleocytoplasmic transport [3].
• Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies [3].
• These MAbs specifically recognized Nup62/p62 as evidenced by immunoblotting analysis using a nuclear membrane fraction [3].

Biological context of Nup62

• The antibodies described in this study provide the means for immunochemical analyses of the NPC protein Nup62/p62 in mammalian cells, and represent useful molecular tools that should permit a better understanding of the biological roles and cellular dynamics of this protein in nucleocytoplasmic transport, cell division, and nuclear organization [3].

Analytical, diagnostic and therapeutic context of Nup62

• Western blotting of total brain homogenates, using the same antibody, shows a p39 band in control rats which is accompanied by a strong, phosphorylated p62 double-band in irradiated animals [4].

References