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Gene Review:

p143 - ORF70 helicase

Spodoptera exigua MNPV

Croizier, G. et al., Argaud, O. et al., Heldens, J.G. et al., Passarelli, A.L. et al., Ramirez Gaite, M.

Marta Ramirez Gaite,

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Disease relevance of p143

Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene [1].
To determine the mutational events in the p143 gene required to allow AcMNPV replication in B. mori cells, AcMNPV recombinants produced in Sf9 cells were screened in vivo in B. mori larvae, which are more permissive to baculovirus infection than B. mori cell lines [2].

High impact information on p143

Replacement in the AcNPV genome of three AcNPV-specific amino acids by the three corresponding BmNPV-specific amino acids at positions 556, 564, and 577 of the p143 protein extends AcNPV host range to B. mori larvae [1].
This protein had considerable amino acid sequence similarity (58%) to AcMNPV p143, including seven conserved motifs characteristic of helicases [3].
One gene, p143, was previously shown to be essential for viral DNA replication and shares sequence motifs with DNA helicases [4] [5].

References

Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene. Croizier, G., Croizier, L., Argaud, O., Poudevigne, D. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
Two key mutations in the host-range specificity domain of the p143 gene of Autographa californica nucleopolyhedrovirus are required to kill Bombyx mori larvae. Argaud, O., Croizier, L., López-Ferber, M., Croizier, G. J. Gen. Virol. (1998) [Pubmed]
Characterization of a putative Spodoptera exigua multicapsid nucleopolyhedrovirus helicase gene. Heldens, J.G., Liu, Y., Zuidema, D., Goldbach, R.W., Vlak, J.M. J. Gen. Virol. (1997) [Pubmed]
Site-directed mutagenesis of the AcMNPV p143 gene: effects on baculovirus DNA replication. Liu, G., Carstens, E.B. Virology. (1999) [Pubmed]
Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. Lu, A., Carstens, E.B. Virology. (1991) [Pubmed]

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