Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

traM - conjugal transfer protein TraM

Escherichia coli

Lu, J. et al., Beranek, A. et al., Disqué-Kochem, C. et al., Vannini, A. et al.

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Disease relevance of traM

Crystallization and preliminary X-ray diffraction studies of the quorum-sensing regulator TraM from Agrobacterium tumefaciens [1].

High impact information on traM

TraM is essential for F plasmid-mediated bacterial conjugation, where it binds to the plasmid DNA near the origin of transfer, and recognizes a component of the transmembrane DNA transfer complex, TraD [2].
Comparison of TraM to its Glu88 mutants predicted to stabilize the helical structure suggests that the protonated state is the active form for binding TraD in conjugation [2].
In this report we present in vitro and in vivo data that demonstrate specific binding of CP TraD of the IncFII R1 plasmid transfer system to relaxosomal protein TraM [3].
The cytoplasmic protein TraM is one of four essential gene products of the F factor which are involved in DNA transfer after mating pair formation [4].

Analytical, diagnostic and therapeutic context of traM

With overlay assays and enzyme-linked immunosorbent assays we showed that a truncated version of TraD, termed TraD11 (DeltaN155), interacted strongly with TraM [3].

References

Crystallization and preliminary X-ray diffraction studies of the quorum-sensing regulator TraM from Agrobacterium tumefaciens. Vannini, A., Volpari, C., Di Marco, S. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM. Lu, J., Edwards, R.A., Wong, J.J., Manchak, J., Scott, P.G., Frost, L.S., Glover, J.N. EMBO J. (2006) [Pubmed]
Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the substrate selector protein TraM. Beranek, A., Zettl, M., Lorenzoni, K., Schauer, A., Manhart, M., Koraimann, G. J. Bacteriol. (2004) [Pubmed]
The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. Disqué-Kochem, C., Dreiseikelmann, B. J. Bacteriol. (1997) [Pubmed]

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