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Gene Review

rps7  -  30S ribosomal protein S7

Chlamydomonas reinhardtii

 
 
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Disease relevance of rps7

  • Five point mutants in the rps7 5'UTR were selected on the basis of their failure to accumulate reporter mRNA in Escherichia coli [1].
 

High impact information on rps7

  • Certain mutations isolated in the 5' untranslated region (5'UTR) of the chloroplast rps7 gene in Chlamydomonas reduce expression of reporter genes [2].
  • The 20-kD protein was shown to be the S7 protein of the chloroplast ribosomal small subunit encoded by rps7, whereas the 47-kD protein was shown to be RB47, a poly(A) binding protein [2].
  • Twelve single base pair changes were found at different positions in the rps7 5'UTR in 207 white colonies examined [3].
  • Cis-acting sequences within the 5'-UTRs of two chloroplast mRNAs (rps7 and atpB) have been identified which are protected from digestion by RNase T1 by extracts enriched for the 81-, 47-, and 38-kDa proteins [4].
  • However, rps12 of C. reinhardtii is no longer immediately adjacent to the rps7 gene encoding ribosomal protein S7 as it is in other organisms [5].
 

Biological context of rps7

  • Only the 5' portion of this gene is located in the opposite unique sequence region of the C. reinhardtii chloroplast genome where the rps7 gene was previously mapped by heterologous hybridization [6].
  • DNA sequence analysis of this region revealed a 159 bp open reading frame corresponding to the 3' half of the rps7 gene, encoding the S7 protein of the small subunit of the chloroplast ribosome [6].
  • Cotranscription of the wild-type chloroplast atpE gene encoding the CF1/CF0 epsilon subunit with the 3' half of the rps7 gene in Chlamydomonas reinhardtii and characterization of frameshift mutations in atpE [6].

References

  1. Mutations that alter the higher-order structure of its 5' untranslated region affect the stability of chloroplast rps7 mRNA. Fargo, D.C., Hu, E., Boynton, J.E., Gillham, N.W. Mol. Gen. Genet. (2000) [Pubmed]
  2. Chloroplast ribosomal protein S7 of Chlamydomonas binds to chloroplast mRNA leader sequences and may be involved in translation initiation. Fargo, D.C., Boynton, J.E., Gillham, N.W. Plant Cell (2001) [Pubmed]
  3. Mutations altering the predicted secondary structure of a chloroplast 5' untranslated region affect its physical and biochemical properties as well as its ability to promote translation of reporter mRNAs both in the Chlamydomonas reinhardtii chloroplast and in Escherichia coli. Fargo, D.C., Boynton, J.E., Gillham, N.W. Mol. Cell. Biol. (1999) [Pubmed]
  4. Translational regulation of chloroplast genes. Proteins binding to the 5'-untranslated regions of chloroplast mRNAs in Chlamydomonas reinhardtii. Hauser, C.R., Gillham, N.W., Boynton, J.E. J. Biol. Chem. (1996) [Pubmed]
  5. Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli. Liu, X.Q., Gillham, N.W., Boynton, J.E. J. Biol. Chem. (1989) [Pubmed]
  6. Cotranscription of the wild-type chloroplast atpE gene encoding the CF1/CF0 epsilon subunit with the 3' half of the rps7 gene in Chlamydomonas reinhardtii and characterization of frameshift mutations in atpE. Robertson, D., Boynton, J.E., Gillham, N.W. Mol. Gen. Genet. (1990) [Pubmed]
 
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