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BPSS1954  -  polymerase

Burkholderia pseudomallei K96243

 
 
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Disease relevance of BPSS1954

  • Short report: application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis [1].
  • Through overlapping extension polymerase chain reaction, the heavy and light chain fragments were linked to form the ScFv which was subsequently cloned into the phage display vector and transformed into ER2537 cells to yield a complexity of 10(8) clones [2].
 

High impact information on BPSS1954

  • A cosmid clone containing DNA with homology to five TTS genes was sub-cloned and regions were sequenced in order to design oligonucleotides for polymerase chain reaction assays [3].
 

Analytical, diagnostic and therapeutic context of BPSS1954

References

  1. Short report: application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. Gal, D., Mayo, M., Spencer, E., Cheng, A.C., Currie, B.J. Am. J. Trop. Med. Hyg. (2005) [Pubmed]
  2. Phage display of recombinant antibodies toward Burkholderia pseudomallei exotoxin. Nathan, S., Li, H., Mohamed, R., Embi, N. J. Biochem. Mol. Biol. Biophys. (2002) [Pubmed]
  3. A putative type III secretion gene cluster is widely distributed in the Burkholderia cepacia complex but absent from genomovar I. Parsons, Y.N., Glendinning, K.J., Thornton, V., Hales, B.A., Hart, C.A., Winstanley, C. FEMS Microbiol. Lett. (2001) [Pubmed]
  4. Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction. Brook, M.D., Currie, B., Desmarchelier, P.M. J. Appl. Microbiol. (1997) [Pubmed]
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