Disease relevance of repA

• Upstream and divergently transcribed from repA, an ORF that showed significant (48 to 64%) homology to a number of proteins that are required for faithful segregation of chromosomal or plasmid DNA of gram-negative bacteria was identified [1].
• A Bacillus subtilis strain was constructed that contained the repA gene of the lactococcal plasmid pWVO1 in its chromosome [2].

High impact information on repA

• The results also suggest that the origin of replication is located outside repA [1].
• A lactococcal plasmid plus origin and two replication protein-coding regions (repA and repB) were located [3].
• An improved version of this thermosensitive replicon was obtained by fusioning the replication repA gene with the downstream repB gene [4].
• In vivo substitution of the native repB promoter sequence with a Tn5-derived promoter sequence was demonstrated. repA, a 344-bp cis-acting region which is the probable pCI305 replication origin region, was noncoding, was AT-rich, and possessed a unique set of inverted and direct repeat sequences [5].

Biological context of repA

• The transcribed inverted repeat sequence between repA and repB could form an attenuator to regulate pFX2 replication [3].

References