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Gene Review:

NGO0055 - pilus-associated protein

Neisseria gonorrhoeae FA 1090

Graupner, S. et al., Wolfgang, M. et al., Drake, S.L. et al., Parge, H.E. et al., Winther-Larsen, H.C. et al.

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Disease relevance of NGO0055

Neisseria gonorrhoeae PilV, a type IV pilus-associated protein essential to human epithelial cell adherence [1].
The mutant was hyperpiliated but defective for further pilus-associated properties, such as twitching motility and plating of pilus-specific phage PO4 [2].

High impact information on NGO0055

Here, we show that the defect in pilus biogenesis resulting from mutations in the pilC gene, encoding a putative pilus-associated adhesin for human tissue, can be suppressed by the absence of functional PilT [3].
Gonococcal mutants bearing transposon insertions in these ORFs were non-piliated and failed to express pilus-associated phenotypes, and the corresponding genes were designated PilO and pilP [4].

Analytical, diagnostic and therapeutic context of NGO0055

Removal of pilus-associated proteins and stabilization of pilin multimers permitted the reproducible crystallization of pilin [5].

References

Neisseria gonorrhoeae PilV, a type IV pilus-associated protein essential to human epithelial cell adherence. Winther-Larsen, H.C., Hegge, F.T., Wolfgang, M., Hayes, S.F., van Putten, J.P., Koomey, M. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI. Graupner, S., Weger, N., Sohni, M., Wackernagel, W. J. Bacteriol. (2001) [Pubmed]
Suppression of an absolute defect in type IV pilus biogenesis by loss-of-function mutations in pilT, a twitching motility gene in Neisseria gonorrhoeae. Wolfgang, M., Park, H.S., Hayes, S.F., van Putten, J.P., Koomey, M. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
PilP, a pilus biogenesis lipoprotein in Neisseria gonorrhoeae, affects expression of PilQ as a high-molecular-mass multimer. Drake, S.L., Sandstedt, S.A., Koomey, M. Mol. Microbiol. (1997) [Pubmed]
Biochemical purification and crystallographic characterization of the fiber-forming protein pilin from Neisseria gonorrhoeae. Parge, H.E., Bernstein, S.L., Deal, C.D., McRee, D.E., Christensen, D., Capozza, M.A., Kays, B.W., Fieser, T.M., Draper, D., So, M. J. Biol. Chem. (1990) [Pubmed]

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