Disease relevance of lysE

• lysE24 is an allele of lysE encoding an L-lysine exporter of Corynebacterium glutamicum [1].

High impact information on lysE

• A probable regulatory gene, lysG, is localized immediately adjacent to lysE and displays all the typical structural features of an autoregulatory transcriptional regulator of the LysR-type family [2].
• L-Lysine export is correlated with lysE expression [2].
• Although L-histidine and L-citrulline also act as coinducers of lysE expression, these two amino acids are not exported by LysE [3].
• The transformant also possessed increased tolerance to S-(2-aminoethyl)-l-cysteine (an l-lysine analog). lysE24 has a single-base insertion mutation in the middle of the lysE gene, and its product is presumably quite different in structure from wild-type LysE [4].
• We were unable to stably transform M. methylotrophus with a plasmid expressing the wild type lysE gene, but happened to obtain a transformant carrying a spontaneously mutated lysE gene (designated lysE24) which could induce l-lysine production even in the wild type strain [4].

Biological context of lysE

• Here, the transcript initiation site of lysE, and its divergently transcribed regulator gene, lysG, are identified [3].
• Although lysE24 has a mutation in the middle of lysE that results in chain termination, the entire lysE locus, including the region downstream of the short open reading frame, is necessary for L-lysine production [1].

Analytical, diagnostic and therapeutic context of lysE

• Single-copy transcriptional fusions of lysE with lacZ, and titration experiments, show that LysG is the positive regulator of lysE expression enabling its up to 20-fold induction [3].

References