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Gene Review:

SR - sepiapterin reductase

Dictyostelium discoideum AX4

Kim, Y.A. et al., Choi, Y.K. et al.

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Disease relevance of sprA

Characterization of recombinant Dictyostelium discoideum sepiapterin reductase expressed in E. coli [1].

High impact information on sprA

The mutant cells, being completely devoid of SR protein, showed 18.1% of L-erythro-tetrahydrobiopterin (BH4) and 0.6% of DH4 productions in the wild type cells [2].
Furthermore, in a coupled assay with Dictyostelium SR, the mutant cell extract exhibited a novel enzyme activity converting 6-pyruvoyltetrahydropterin to 1'-oxo-2'-D-hydroxypropyl-H4-pterin [2].
The purified His-tag recombinant enzyme was confirmed to have the genuine activity of SR to produce tetrahydrobiopterin from 6-pyruvoyltetrahydropterin in a coupled assay with 6-pyruvoyltetrahydropterin synthase as well as dihydrobiopterin from sepiapterin [1].
A cDNA clone (SSC801) putatively encoding sepiapterin reductase (SR) was obtained from the expressed sequence tag clones of Dictyostelium discoideum [1].
The deduced amino acid sequence showed 29.8% identity with mouse SR sequence and a molecular mass of 29,969 Da [1].

References

Characterization of recombinant Dictyostelium discoideum sepiapterin reductase expressed in E. coli. Kim, Y.A., Chung, H.J., Kim, Y.J., Choi, Y.K., Hwang, Y.K., Lee, S.W., Park, Y.S. Mol. Cells (2000) [Pubmed]
D-threo-tetrahydrobiopterin is synthesized via 1'-oxo-2'-D-hydroxypropyl-tetrahydropterin in Dictyostelium discoideum Ax2. Choi, Y.K., Park, J.S., Kong, J.S., Morio, T., Park, Y.S. FEBS Lett. (2005) [Pubmed]

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