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Gene Review:

kluB - plasmid stability protein

IncP-1beta multiresistance plasmid pB8

Easter, C.L. et al., Deane, S.M. et al., Heuer, H. et al., Karsi, A. et al., Jagura-Burdzy, G. et al., et al.

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Disease relevance of kluB

To further define the role of this region in promoting the stable maintenance of plasmid RK2, the parCBA and parDE operons separately and the intact (parCBA/DE) par region (3.2 kb) were reintroduced into an RK2 plasmid deleted for par and assayed for plasmid stability in two Escherichia coli strains (MC1061K and MV10delta lac) [1].
The resulting plasmid, pHUTFXPAR, conferred the plasmid stability, trifolitoxin production, and H2 uptake phenotypes in the broad-host-range organism Sinorhizobium sp. strain ANU280 [2].
We determined if the IncP-1beta plasmid pB10 was unstable in some Proteobacteria, and whether plasmid stability was enhanced after long-term carriage in a single host and when regularly switched between isogenic hosts [3].
Effect of parB on plasmid stability and gene expression in Xanthomonas campestris [4].
Edwardsiella ictaluri strain 93-146 transformed with the new plasmid, pAKlux1, was highly bioluminescent. pAKlux1 was stably maintained in E. ictaluri without any apparent effect on growth or native plasmid stability [5].

High impact information on kluB

The korABF operon of broad host range IncP plasmid RK2 encodes proteins that coordinate expression of many other operons and that aid plasmid stability by providing at least part of a partitioning apparatus [6].
Plasmid pTC-F14 contains a plasmid stability system called pas (plasmid addiction system), which consists of two proteins, a PasA antitoxin and a PasB toxin [7].
Plasmid curing resulted in the arrest of cell growth but did not cause cell death, and plasmid stability was not influenced by the E. coli mazEF genes [7].

References

Contribution of different segments of the par region to stable maintenance of the broad-host-range plasmid RK2. Easter, C.L., Sobecky, P.A., Helinski, D.R. J. Bacteriol. (1997) [Pubmed]
A transposable partitioning locus used to stabilize plasmid-borne hydrogen oxidation and trifolitoxin production genes in a Sinorhizobium strain. Kent, A.D., Wojtasiak, M.L., Robleto, E.A., Triplett, E.W. Appl. Environ. Microbiol. (1998) [Pubmed]
Frequent conjugative transfer accelerates adaptation of a broad-host-range plasmid to an unfavorable Pseudomonas putida host. Heuer, H., Fox, R.E., Top, E.M. FEMS Microbiol. Ecol. (2007) [Pubmed]
Effect of parB on plasmid stability and gene expression in Xanthomonas campestris. Pimenta, A.d.e. .L., Rosato, Y.B., Astolfi-Filho, S. Lett. Appl. Microbiol. (1992) [Pubmed]
Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring. Karsi, A., Menanteau-Ledouble, S., Lawrence, M.L. FEMS Microbiol. Lett. (2006) [Pubmed]
kfrA gene of broad host range plasmid RK2 encodes a novel DNA-binding protein. Jagura-Burdzy, G., Thomas, C.M. J. Mol. Biol. (1992) [Pubmed]
Plasmid evolution and interaction between the plasmid addiction stability systems of two related broad-host-range IncQ-like plasmids. Deane, S.M., Rawlings, D.E. J. Bacteriol. (2004) [Pubmed]

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