Disease relevance of N

• This ORF was similar in size to the N protein ORFs of other rhabdoviruses [1].
• Among them, a sequence of 24 amino acids which was similar between LNYV and SYNV N proteins corresponded to a region with high homology between isolates of vesicular stomatitis virus and rabies virus N protein sequences, the type members of the genera Vesiculovirus and Lyssavirus of animal rhabdoviruses, respectively [1].

High impact information on N

• However, little if any direct sequence homology was found with N protein sequences of other rhabdoviruses [1].
• Short amino acid sequences were found to be conserved between the N proteins of LNYV and sonchus yellow net virus (SYNV), a member of the genus Nucleorhabdovirus of plant rhabdoviruses [1].
• The previously identified N gene was adjacent to the 3' leader RNA and began at position 91 from the 3' end of the LNYV genome [1].
• The complete nucleocapsid (N) genes of eight Australian isolates of Lettuce necrotic yellows virus (LNYV) were amplified by reverse transcription PCR, cloned and sequenced [2].
• A procedure is described for the detection of lettuce necrotic yellows virus (LNYV) nucleocapsid protein (N) or envelope glycoprotein (G) by immuno-blotting with their respective monoclonal antibodies [3].

Biological context of N

• Analysis of the sequence showed that the N mRNA contained a 78-nt 5' untranslated region, followed by a 1377-nt open reading frame (ORF) encoding a 459-amino-acid protein [1].

References