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Gene Review

I-2  -  Inhibitor-2

Drosophila melanogaster

Synonyms: CG10574, DI-2, Dm I-2, Dmel\CG10574, I-2Dm, ...
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High impact information on I-2

  • Here we show by expression of cDNA in bacteria, that PPY is a protein serine phosphatase and that its biochemical properties are distinct from PP1 in both substrate specificity and regulation by the thermostable inhibitory proteins inhibitor 1 and inhibitor 2 [1].
  • I-4 inhibited activity of the catalytic subunit of PP1 (PP1C), specifically with an IC(50) of 0.2 nM, more potently than I-2 with an IC(50) of 2 nM [2].
  • Phosphatase inhibitor-2 (I-2) is a mammalian phosphoprotein that binds to the catalytic subunit of type 1 serine/threonine phosphoprotein phosphatase (PP1c) and inhibits its activity in vitro [3].
  • Inhibitor-2 partially purified from Drosophila had an identical molecular mass to its mammalian counterpart, and recombined with mammalian protein phosphatase-1 to form a hybrid protein phosphatase-1I [4].

Biological context of I-2

  • The sequence of I-2Dm is 35% identical with that of I-2, whereas the catalytic subunits themselves are >85% identical in flies and humans; however, we show that many biochemical properties of I-2 are conserved [3].


  1. Drosophila PPY, a novel male specific protein serine/threonine phosphatase localised in somatic cells of the testis. Armstrong, C.G., Mann, D.J., Berndt, N., Cohen, P.T. J. Cell. Sci. (1995) [Pubmed]
  2. Identification and characterization of a novel protein inhibitor of type 1 protein phosphatase. Shirato, H., Shima, H., Sakashita, G., Nakano, T., Ito, M., Lee, E.Y., Kikuchi, K. Biochemistry (2000) [Pubmed]
  3. The chaperone-like properties of mammalian inhibitor-2 are conserved in a Drosophila homologue. Bennett, D., Szöor, B., Alphey, L. Biochemistry (1999) [Pubmed]
  4. The protein phosphatases of Drosophila melanogaster and their inhibitors. Orgad, S., Dudai, Y., Cohen, P. Eur. J. Biochem. (1987) [Pubmed]
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